Background: Semaphorin 3F (SEMA3F) plays a substantial role in carcinogenesis, because of its role in inducing angiogenesis, and creating a microenvironment for the developing tumor.

Objective: The purpose of this work was to assess the impact of cisplatin, depending on the concentration and exposure time on the expression pattern of SEMA3F in an endometrial cancer cell line.

Materials and Methods: Cultures of the Ishikawa endometrial cancer cells were incubated with cisplatin with the following concentrations: 2.5μM; 5μM; and 10μM and for the following periods of time: 12; 24; and 48 hours. Cells not incubated with the drug constituted the control in the experiment. To determine the effect of cisplatin on the expression of SEMA3F, the real-time quantitative reverse transcription reaction (RtqPCR; mRNA) was used, as well as the ELISA assay (protein). The statistical analysis was done with the admission of p<0.05.

Results: The silencing of SEMA3F expression on the transcriptome and proteome levels in a culture unexposed to the effects of cisplatin in comparison to endometrial cancer cells under the influence of cisplatin (p<0.05) were noted. Along with an increase in the concentration of the drug used, the number of copies of the gene transcript, during the shortest incubation period had a gradual increase. Only for the highest concentration of the drug, substantial statistical differences in the expression of the SEMA3F protein between 24 and 48 hour incubation periods (p<0.05) were determined.

Conclusion: Using cisplatin in an endometrial cancer cell culture results in an increased expression of SEMA3F, which advantageously affects the normalization of the neoplastic angiogenic process and lowers the proliferation of the cells making up the mass of the tumor.

背景：Semaphorin 3F（SEMA3F）在致癌作用中起着重要作用，因为它在诱导血管生成和为发展中的肿瘤创造微环境中起着重要的作用。

目的：这项工作的目的是评估顺铂的浓度和暴露时间对子宫内膜癌细胞株SEMA3F表达模式的影响。

材料与方法：将石川子宫内膜癌细胞培养物与顺铂孵育，其浓度为2.5μM。5微米 在以下时间段内为10μM：12；24; 和48小时。未与药物孵育的细胞构成实验中的对照。为了确定顺铂对SEMA3F表达的影响，使用了实时定量逆转录反应（RtqPCR； mRNA）以及ELISA测定法（蛋白质）。统计学分析以p ＜0.05为准。

结果：与顺铂作用下的子宫内膜癌细胞相比，注意到未暴露于顺铂作用的培养物中SEMA3F表达对转录组和蛋白质组水平的沉默（p <0.05）。随着使用药物浓度的增加，在最短的温育期内，基因转录本的拷贝数逐渐增加。仅对于最高浓度的药物，在24和48小时的孵育时间段内，SEMA3F蛋白的表达存在显着的统计学差异（p <0.05）。

结论：在子宫内膜癌细胞培养物中使用顺铂可导致SEMA3F的表达增加，这有利于影响肿瘤血管生成过程的正常化，并降低构成肿瘤块的细胞的增殖。