Objectives: To investigate the effect of Danggui-Shaoyao-San (DSS)-containing serum on the renal tubular Epithelial-Mesenchymal Transition (EMT) of Diabetic Nephropathy (DN) in high glucose- induced HK-2 cells and its mechanism.

Methods: 20 rats were randomly divided into four groups: blank control group, DSS low dose group (DSS-L), DSS middle dose group (DSS-M), and DSS high dose group (DSS-H). DSS was administrated to the corresponding group (7g/kg/d, 14g/kg/d and 21g/kg/d) for 7 consecutive days, and the same volume of saline was given to the blank control group by gavage. The rat drug-containing serum was successfully prepared. HK-2 cells were divided into five groups: blank control group, model group, DSS-L, DSS-M, DSS-H, according to the corresponding drug and dose of each treatment group. Protein and mRNA levels of Jagged1, Notch1, Hes5, Notch Intracellular Domain (NICD), E-cadherin, alpha- Smooth Muscle Actin (α-SMA) and vimentin at 24h, 48h and 72h were detected by Western Blot and RT-qPCR.

Results: The protein and mRNA levels of Jagged1, Notch1, Hes5, NICD, α-SMA and vimentin in the treatment groups were remarkably decreased compared with the model group (P<0.05), and the protein and mRNA levels of E-cadherin were notably increased (P<0.05) by Western Blot and RT-qPCR.

Conclusion: Our results demonstrated that DSS could prevent DN by ameliorating renal tubular EMT through inhibition of the Notch signaling pathway.

目的：研究当归-Sha药散（DSS）血清对高糖诱导的HK-2细胞肾小管上皮-间质转化（EMT）的影响及其机制。

方法：20只大鼠随机分为四组：空白对照组，DSS低剂量组（DSS-L），DSS中剂量组（DSS-M）和DSS高剂量组（DSS-H）。将DSS连续7天施用于相应的组（7g / kg / d，14g / kg / d和21g / kg / d），并且通过管饲法将相同体积的盐水给予空白对照组。成功制备了含大鼠药物的血清。根据每个治疗组的相应药物和剂量，将HK-2细胞分为五组：空白对照组，模型组，DSS-L，DSS-M，DSS-H。通过Western Blot和RT-qPCR检测Jagged1，Notch1，Hes5，Notch细胞内结构域（NICD），E-钙黏着蛋白，α-平滑肌肌动蛋白（α-SMA）和波形蛋白的蛋白和mRNA水平。

结果：与模型组相比，治疗组Jagged1，Notch1，Hes5，NICD，α-SMA和波形蛋白的蛋白和mRNA水平明显降低（P <0.05），E-钙粘蛋白的蛋白和mRNA水平较模型组明显降低。 Western Blot和RT-qPCR显着增加（P <0.05）。

结论：我们的结果表明，DSS可以通过抑制Notch信号通路来改善肾小管EMT，从而预防DN。