Biomaterials regulate macrophages and promote regeneration function, which is a new hot pot in tissue engineering and regenerative medicine. The research based on macrophage materials biology has appeared happy future, but related research on regulating macrophages and promoting tissue regeneration is still in its infancy. The surface roughness of biomaterials is one of the important factors affecting macrophage behavior. Previous study also found that the surface roughness of many biomaterials regulating macrophage polarization, but not including mineralized collagen (MC). In this study, we designed and fabricated MC with different roughness and investigated the influence of MC with different roughness on macrophages. In the study, we found that on the rough surface of MC, macrophages exhibited M1 phenotype-amoeboid morphology and high-level secretory of inflammatory factor (tumor necrosis factor-α and interleukin-6), while smoother surface exhibited M2 phenotype. These data will be beneficial to understand the mechanism deeply and enrich biomaterials tissue regeneration theory, provide a new train of thought biomaterials inducing tissue regeneration and repair and guide the optimum design of new biomaterials, development and reasonable applications.

中文翻译：

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在矿化胶原蛋白的不同表面粗糙度下观察到的巨噬细胞表型差异。

生物材料调节巨噬细胞并促进再生功能，这是组织工程和再生医学中的新热点。基于巨噬细胞材料生物学的研究似乎前景广阔，但有关调节巨噬细胞和促进组织再生的研究仍处于起步阶段。生物材料的表面粗糙度是影响巨噬细胞行为的重要因素之一。先前的研究还发现，许多生物材料调节巨噬细胞极化的表面粗糙度，但不包括矿化胶原蛋白（MC）。在这项研究中，我们设计和制造了具有不同粗糙度的MC，并研究了具有不同粗糙度的MC对巨噬细胞的影响。在研究中，我们发现在MC的粗糙表面上，巨噬细胞表现出M1表型-类腺样形态和炎性因子（肿瘤坏死因子-α和白介素-6）的高水平分泌，而更光滑的表面表现出M2表型。这些数据将有助于深入了解生物材料的组织再生机理，为生物材料诱导组织再生和修复提供新思路，指导新生物材料的优化设计，开发和合理应用。