Methacrylated gelatin (GelMA)/bacterial cellulose (BC) composite hydrogels have been successfully prepared by immersing BC particles in GelMA solution followed by photo-crosslinking. The morphology of GelMA/BC hydrogel was examined by scanning electron microscopy and compared with pure GelMA. The hydrogels had very well interconnected porous network structure, and the pore size decreased from 200 to 10 µm with the increase of BC content. The composite hydrogels were also characterized by swelling experiment, X-ray diffraction, thermogravimetric analysis, rheology experiment and compressive test. The composite hydrogels showed significantly improved mechanical properties compared with pure GelMA. In addition, the biocompatility of composite hydrogels were preliminarily evaluated using human articular chondrocytes. The cells encapsulated within the composite hydrogels for 7 days proliferated and maintained the chondrocytic phenotype. Thus, the GelMA/BC composite hydrogels might be useful for cartilage tissue engineering.

中文翻译：

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用于软骨组织工程的甲基丙烯酸明胶/细菌纤维素复合水凝胶的制备和表征。

通过将BC颗粒浸入GelMA溶液中并进行光交联，成功制备了甲基丙烯酸明胶（GelMA）/细菌纤维素（BC）复合水凝胶。通过扫描电子显微镜检查 GelMA/BC 水凝胶的形态，并与纯 GelMA 进行比较。水凝胶具有良好互连的多孔网络结构，随着BC含量的增加，孔径从200μm减小到10μm。还通过溶胀实验、X射线衍射、热重分析、流变实验和压缩实验对复合水凝胶进行了表征。与纯 GelMA 相比，复合水凝胶的机械性能显着提高。此外，利用人关节软骨细胞初步评估了复合水凝胶的生物相容性。封装在复合水凝胶内 7 天的细胞增殖并维持软骨细胞表型。因此，GelMA/BC 复合水凝胶可能可用于软骨组织工程。