NO is the earliest discovered gas signal molecule which is produced by normal healthy endothelial cells, and it has many functions, such as maintaining cardiovascular homeostasis, regulating vasodilation, inhibiting intimal hyperplasia and preventing atherosclerosis in the blood system. Insufficient NO release is often observed in the pathological environment, for instance atherosclerosis. It was discovered that NO could be released from the human endogenous NO donor by many compounds, and these methods can be used for the treatment of certain diseases in the blood system. In this work, a series of copper-loaded polydopamine (PDA) coatings were produced through self-polymerization time for 24, 48 and 72 h. The chemical composition and structure, coating thickness and hydrophilicity of the different copper-loaded PDA coatings surfaces were characterized by phenol hydroxyl quantitative, X-ray photoelectron spectroscopy, ellipsometry atomic force microscopy and water contact angles. The results indicate that the thickness and the surface phenolic hydroxyl density of the PDA coatings increased with the polymerization time.This copper-loaded coating has glutathione peroxidase-like activity, and it has the capability of catalyzing NO releasing from GSNO. The surface of the coating showed desirable hemocompatibility, the adhesion and activation of platelets were inhibited on the copper-loaded coatings. At the same time, the formation of the thrombosis was also suppressed. These copper-loaded PDA coatings could provide a promising platform for the development of blood contact materials.

中文翻译：

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负载 Cu∥ 的聚多巴胺涂层具有原位一氧化氮生成功能，可改善血液相容性。

NO是最早发现的气体信号分子，由正常健康的内皮细胞产生，具有维持心血管稳态、调节血管舒张、抑制内膜增生、预防血液系统动脉粥样硬化等多种功能。在病理环境中，例如动脉粥样硬化，经常观察到 NO 释放不足。人们发现许多化合物可以从人体内源性NO供体中释放NO，这些方法可用于治疗某些血液系统疾病。在这项工作中，通过24、48和72小时的自聚合时间制备了一系列载铜聚多巴胺（PDA）涂层。采用酚羟基定量、X射线光电子能谱、椭圆光度原子力显微镜和水接触角等手段对不同载铜PDA涂层表面的化学成分和结构、涂层厚度和亲水性进行了表征。结果表明，随着聚合时间的延长，PDA涂层的厚度和表面酚羟基密度增加。该载铜涂层具有类谷胱甘肽过氧化物酶活性，具有催化GSNO释放NO的能力。涂层表面表现出良好的血液相容性，载铜涂层上血小板的粘附和活化受到抑制。同时，血栓的形成也受到抑制。这些载铜 PDA 涂层可以为血液接触材料的开发提供一个有前途的平台。