Both laboratory and field respirometry are rapidly growing techniques to determine animal performance thresholds. However, replicating protocols to estimate maximum metabolic rate (MMR) between species, populations, and individuals can be difficult, especially in the field. We therefore evaluated seven different exercise treatments-four laboratory methods involving a swim tunnel (critical swim speed [Ucrit], Ucrit postswim fatigue, maximum swim speed [Umax], and Umax postswim fatigue) and three field-based chasing methods (3-min chase with 1-min air exposure, 3-min chase with no air exposure, and chase to exhaustion)-in adult coho salmon (Oncorhynchus kisutch) as a case study to determine best general practices for measuring and quantifying MMR in fish. We found that all seven methods were highly comparable and that chase treatments represent a valuable field alternative to swim tunnels. Moreover, we caution that the type of test and duration of measurement windows used to calculate MMR can have significant effects on estimates of MMR and statistical power for each approach.

中文翻译：

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最大化：优化鱼类最大代谢率现场测量的准确性、精度和功率。

实验室和现场呼吸测量法都是快速发展的确定动物性能阈值的技术。然而，复制估计物种、种群和个体之间最大代谢率 (MMR) 的方案可能很困难，尤其是在野外。因此，我们评估了七种不同的运动治疗方法——四种涉及泳道的实验室方法（临界游泳速度 [Ucrit]、Ucrit 游后疲劳、最大游泳速度 [Umax] 和 Umax 游后疲劳）和三种基于现场的追逐方法（3 分钟以成年银大麻哈鱼 (Oncorhynchus kisutch) 为案例研究，确定测量和量化鱼类 MMR 的最佳一般做法。我们发现所有七种方法都具有高度可比性，并且追逐治疗是游泳隧道的一种有价值的现场替代方法。此外，我们提醒您，用于计算 MMR 的测试类型和测量窗口持续时间可能会对每种方法的 MMR 估计和统计功效产生重大影响。