In traditional Chinese medicine (TCM) studies, it is difficult to choose evaluation markers for the strict quality control of herbs. A high performance liquid chromatography coupled with metabolomics for simultaneous quantitative analysis of quality markers (Q-markers) in Glycyrrhiza uralensis Fisch was established, which could not only ensure the quality and batch-to-batch consistency of TCMs, but also achieve a quantitative analysis of multi-components by the single reference standard. Based on the construction of chromatographic profiles by high performance liquid chromatography (HPLC) and HPLC-Q-Exactive/MS methods, different multivariate analyses were employed. Seven quantitative indices were selected as the Q-markers, and a reliable quantification method was established. The quantitative method was acceptable with good linearity with correlation coefficients >0.9993 and satisfactory repeatability (relative standard deviation (RSD) < 0.05%), precision (RSD < 0.24%), reproducibility (RSD < 0.97%), stability (RSD < 2.52%) and recoveries (96.96%—98.52%, RSD < 3.24%), and no significant differences were observed between the external standard method and the new method as determined by calculating standard method difference. Overall, the study suggests that the simultaneous quantitative analysis of main Q-marker in G. uralensis Fisch with one single marker can be considered good quality criteria for performing quality control of G. uralensis Fisch.

中文翻译：

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甘草甘草中单一参考的Q标记同时定量分析

在中药（TCM）研究中，很难选择评估标记来严格控制草药的质量。高效液相色谱法和代谢组学同时定量分析甘草中的质量标记（Q标记）建立了Fisch，不仅可以确保中药的质量和批次间的一致性，而且可以通过单一参考标准对多组分进行定量分析。基于高效液相色谱（HPLC）和HPLC-Q-Exactive / MS方法构建色谱图的基础，采用了不同的多元分析。选择七个定量指标作为Q标记，建立了可靠的定量方法。定量方法可接受，线性良好，相关系数> 0.9993，重复性令人满意（相对标准偏差（RSD）<0.05％），精密度（RSD <0.24％），重现性（RSD <0.97％），稳定性（RSD <2.52％） ）和回收率（96.96％-98.52％，RSD <3.24％），通过计算标准方法差异确定的外标方法与新方法之间没有显着差异。总体而言，研究表明，对主要Q标记的同时定量分析具有一个单一标记的G.uralensis Fisch可以被视为进行G.uralensis Fisch质量控制的良好质量标准。