This study aimed to determine the effects of Bifidobacterium bifidum TMC3115, Lactobacillus plantarum 45 (LP45) and their combined use on cognitive performance and gut microbiota in APP/PS1 mice. The APP/PS1 mice were randomly divided into four groups: Alzheimer's disease (AD) model group, TMC3115 group [1 × 109 colony forming unit (CFU)], LP45 group (1 × 109 CFU) and a mixture group of TMC3115 (5 × 108 CFU) and LP45 (5 × 108 CFU). The wild-type littermates were chosen as normal control. The mice were sacrificed at the end of 22 weeks after behavioral evaluation. Collected cecum content was analyzed using 16S rRNA sequencing. Combined use of TMC3115 and LP45 significantly increased the times across the platform, time spent in the target quadrant compared with the AD, TMC3115 and LP45 groups in Morris water maze test. Microbiota analysis showed that combined TMC3115 and LP45 supplementation significantly increased observed species and beta diversity, and reversed gut dysbiosis by decreasing the abundance of Bacteroides and increasing the abundance of Acetatifactor and Millionella. These results indicate the long-term combined administration of TMC3115 and LP45 can improve spatial memory impairment in APP/PS1 mice and suggest that modifying the gut microbiome may provide potential benefits for AD patients.

中文翻译：

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双歧杆菌 TMC3115 和植物乳杆菌 45 的长期联合给药可减轻 APP/PS1 小鼠的空间记忆障碍和肠道菌群失调。

本研究旨在确定双歧杆菌 TMC3115、植物乳杆菌 45 (LP45) 及其组合对 APP/PS1 小鼠认知能力和肠道微生物群的影响。APP/PS1小鼠随机分为四组：阿尔茨海默病（AD）模型组、TMC3115组[1×109菌落形成单位（CFU）]、LP45组（1×109 CFU）和TMC3115（5 × 108 CFU) 和 LP45 (5 × 108 CFU)。选择野生型同窝仔作为正常对照。在行为评估后 22 周结束时处死小鼠。使用 16S rRNA 测序分析收集的盲肠内容物。在 Morris 水迷宫测试中，与 AD、TMC3115 和 LP45 组相比，TMC3115 和 LP45 的组合使用显着增加了整个平台的时间，在目标象限中花费的时间。微生物群分析表明，联合补充 TMC3115 和 LP45 显着增加了观察到的物种和 β 多样性，并通过降低拟杆菌属的丰度和增加乙酰化因子和 Millionella 的丰度来逆转肠道菌群失调。这些结果表明，TMC3115 和 LP45 的长期联合给药可以改善 APP/PS1 小鼠的空间记忆障碍，并表明改变肠道微生物组可能为 AD 患者带来潜在的益处。