To comparatively evaluate the performance of the rapid antimicrobial susceptibility testing (AST) system QMAC-dRAST V2.0 and of standard disk diffusion in agar, AST was performed directly from 100 positive blood culture bottles with Gram-negative bacilli. AST results provided by QMAC-dRAST showed 92.9% agreement with disk diffusion method results. Discrepancies observed between results obtained with QMAC-dRAST and disk diffusion method conducted to 10 very major errors (0.8%, S with QMAC-dRAST vs R with disk diffusion method), 40 major errors (3.2%, R vs S, respectively), 15 minor errors (1.2%, S vs I or I vs R, respectively) and 23 very minors errors (1.8%, I vs S or R vs I, respectively). For very major and major errors, in only 36% of the cases did the repeat QMAC-dRAST confirm the initial result, whereas a repeat AST using disk diffusion method confirmed the initial result in 92% of cases. AST results obtained using microdilution in liquid medium confirmed those obtained with QMAC-dRAST and disk diffusion method in 4% and 89%, respectively. Repeatability and reproducibility tests performed on QMAC-dRAST using reference strains showed 94% to 100% of R/S/I categorical agreement.

中文翻译：

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QMAC-dRAST V2.0系统对革兰氏阴性血培养分离物快速抗生素敏感性测试的比较评估。

为了比较评估快速抗菌药敏测试（AST）系统QMAC-dRAST V2.0和标准琼脂圆盘扩散的性能，直接从100个带有革兰氏阴性杆菌的阳性血液培养瓶中进行了AST。QMAC-dRAST提供的AST结果与磁盘扩散法结果显示92.9％的一致性。使用QMAC-dRAST和磁盘扩散方法获得的结果在10个非常主要的误差（0.8％，使用QMAC-dRAST的S与使用磁盘扩散方法的R）之间，40个主要误差（分别为3.2％，R和S）之间观察到差异， 15个小错误（分别为S与I或I与R的1.2％）和23个小错误（分别与I与S或R与I的1.8％）。对于非常重大的错误，只有36％的案例通过重复QMAC-dRAST确认了初始结果，而使用磁盘扩散法进行的AST重复试验则在92％的病例中证实了最初的结果。在液体介质中使用微量稀释获得的AST结果证实了通过QMAC-dRAST和圆盘扩散法获得的AST结果分别为4％和89％。使用参考菌株对QMAC-dRAST进行的重复性和再现性测试显示R / S / I类别一致性为94％至100％。