Compared to terrestrial environments, the oceans harbor a variety of environments, creating higher biodiversity, which gives marine natural products a high occurrence of significant biology and novel chemistry. However, traditional bioassay-guided isolation and purification strategies are severely limiting the discovery of additional novel natural products from the ocean. With an increasing number of marine microorganisms being sequenced, genome mining is gradually becoming a powerful tool to retrieve novel marine natural products. In this review, we have summarized genome mining approaches used to analyze key enzymes of biosynthetic pathways and predict the chemical structure of new gene clusters by introducing successful stories that used genome mining strategy to identify new marine-derived compounds. Furthermore, we also put forward challenges for genome mining techniques and their proposed solutions. The detailed analysis of the genome mining strategy will help researchers to understand this novel technique and its application. With the development of a genome sequence, genome mining strategies will be applied more widely, which will drive rapid development in the field of marine natural product development.

中文翻译：

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基因组挖掘是一种生物技术工具，用于发现新型海洋天然产物。

与陆地环境相比，海洋具有多种环境，创造了更高的生物多样性，这使海洋天然产物大量出现了重要的生物学和新化学方法。然而，传统的生物测定指导的分离和纯化策略严重地限制了从海洋中发现其他新型天然产物的发现。随着越来越多的海洋微生物被测序，基因组挖掘正逐渐成为检索新型海洋天然产物的有力工具。在这篇综述中，我们总结了用于分析生物合成途径关键酶的基因组挖掘方法，并通过介绍成功的故事来预测新基因簇的化学结构，这些成功案例利用基因组挖掘策略来识别新的海洋衍生化合物。此外，我们还对基因组挖掘技术及其提出的解决方案提出了挑战。对基因组挖掘策略的详细分析将有助于研究人员了解这一新技术及其应用。随着基因组序列的发展，基因组挖掘策略将得到更广泛的应用，这将推动海洋天然产物开发领域的快速发展。