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Kisspeptin induces LH release and ovulation in an induced ovulator†.
Biology of Reproduction ( IF 3.6 ) Pub Date : 2020-04-20 , DOI: 10.1093/biolre/ioaa051
Rodrigo A Carrasco 1 , Carlos E Leonardi 1 , Kylie Hutt 1 , Jaswant Singh 1 , Gregg P Adams 1
Affiliation  

Kisspeptin has been implicated in the ovulatory process of several species of spontaneous ovulators but in only one induced ovulator. In contrast, NGF in semen is the principal trigger of ovulation in other species of induced ovulators—camelids. We tested the hypotheses that kisspeptin induces luteinizing hormone (LH) secretion in llamas through a hypothalamic mechanism, and kisspeptin neurons are the target of NGF in its ovulation-inducing pathway. In Experiment 1, llamas were given either NGF, kisspeptin, or saline intravenously, and LH secretion and ovulation were compared among groups. All llamas treated with NGF (5/5) or kisspeptin (5/5) had an elevation of LH blood concentrations after treatment and ovulated, whereas none of the saline group did (0/5). In Experiment 2, llamas were either pretreated with a gonadotropin-releasing hormone (GnRH) receptor antagonist or saline and treated 2 h later with kisspeptin. Llamas pretreated with saline had elevated plasma LH concentrations and ovulated (6/6) whereas llamas pretreated with cetrorelix did not (0/6). In Experiment 3, we evaluated the hypothalamic kisspeptin-GnRH neuronal network by immunohistochemistry. Kisspeptin neurons were detected in the arcuate nucleus, the preoptic area, and the anterior hypothalamus, establishing synaptic contacts with GnRH neurons. We found no colocalization between kisspeptin and NGF receptors by double immunofluorescence. Functional and morphological findings support the concept that kisspeptin is a mediator of the LH secretory pathway in llamas; however, the role of kisspeptins in the NGF ovulation-inducing pathway in camelids remains unclear since NGF receptors were not detected in kisspeptin neurons in the hypothalamus.

中文翻译:

Kisspeptin 在诱导排卵者中诱导 LH 释放和排卵†。

Kisspeptin 与几种自发排卵器的排卵过程有关,但仅与一种诱导排卵器有关。相比之下,精液中的 NGF 是其他诱导排卵物种——骆驼科动物排卵的主要触发因素。我们测试了 Kisspeptin 通过下丘脑机制诱导美洲驼黄体生成素 (LH) 分泌的假设,并且 Kisspeptin 神经元是 NGF 在其促排卵途径中的目标。在实验 1 中,给美洲驼静脉注射 NGF、kisspeptin 或生理盐水,并比较各组之间的 LH 分泌和排卵。所有用 NGF (5/5) 或 Kisspeptin (5/5) 治疗的美洲驼在治疗和排卵后 LH 血液浓度升高,而盐水组没有一个 (0/5)。在实验 2 中,美洲驼用促性腺激素释放激素 (GnRH) 受体拮抗剂或生理盐水预处理,2 小时后用 Kisspeptin 治疗。用盐水预处理的美洲驼血浆 LH 浓度升高并排卵 (6/6),而用西曲瑞克预处理的美洲驼则没有 (0/6)。在实验 3 中,我们通过免疫组织化学评估了下丘脑 Kisspeptin-GnRH 神经元网络。在弓状核、视前区和下丘脑前部检测到 Kisspeptin 神经元,与 GnRH 神经元建立突触接触。我们通过双重免疫荧光发现kisspeptin 和NGF 受体之间没有共定位。功能和形态学发现支持 Kisspeptin 是美洲驼 LH 分泌途径的介质的概念;然而,
更新日期:2020-06-24
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