Abstract The International Maritime Organization (IMO) has adopted a ballast water convention to prevent spread of invasive species with ballast water and with it comes the need for fast and reliable tests to estimate the number of viable organisms found in ballast water. During 2017 and 2018, computer-assisted automatic quantification of plankton was compared with direct microscopic counts. Analysed samples represented simulations of ballast water samples from Denmark. Samples consisted of live plankton monocultures, mixed cultures, or ambient organisms from local seawater. The aim was to validate the performance and efficiency of the automated Motility and Fluorescence Assay (MFA) system BallastWISE, which automatically counts motile and fluorescent organisms in the fractions ≥10 - Standardized counting methods for comparison were completed under microscope. Organisms in the ≥10 - BallastWISE results correlated well with direct microscopic counts, showing promising counting performance and efficiency when it comes to rapid analysis of ballast water plankton samples. Quantification of monoculture and mixed cultures using BallastWISE correlated well with manual viable counts (Tetraselmis suecica in size range ≥ 10 -

中文翻译：

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用于压载水质量监测的动力和荧光分析 (MFA) 的初步验证研究

摘要 国际海事组织 (IMO) 已通过了压载水公约，以防止通过压载水传播入侵物种，因此需要进行快速可靠的测试来估计压载水中发现的活生物体的数量。2017 年和 2018 年期间，将浮游生物的计算机辅助自动量化与直接显微镜计数进行了比较。分析的样品代表来自丹麦的压载水样品的模拟。样本包括活的浮游生物单一培养物、混合培养物或来自当地海水的环境生物。目的是验证自动化运动和荧光检测 (MFA) 系统 BallastWISE 的性能和效率，自动计数≥10 级分中的活动和荧光生物 - 用于比较的标准化计数方法在显微镜下完成。≥10 - BallastWISE 结果中的生物与直接显微计数密切相关，在快速分析压载水浮游生物样品时显示出良好的计数性能和效率。使用 BallastWISE 对单一培养和混合培养的定量与手动活菌计数（大小范围 ≥ 10 - 的 Tetraselmis suecica）相关性良好