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Preliminary verification studies of the motility and fluorescence assay (MFA) for ballast water quality monitoring
Journal of Sea Research ( IF 1.725 ) Pub Date : 2020-04-19 , DOI: 10.1016/j.seares.2020.101889
Mathias Engell Holmstrup; Pia Haecky; Nicholas Blackburn

The International Maritime Organization (IMO) has adopted a ballast water convention to prevent spread of invasive species with ballast water and with it comes the need for fast and reliable tests to estimate the number of viable organisms found in ballast water. During 2017 and 2018, computer-assisted automatic quantification of plankton was compared with direct microscopic counts. Analysed samples represented simulations of ballast water samples from Denmark. Samples consisted of live plankton monocultures, mixed cultures, or ambient organisms from local seawater. The aim was to validate the performance and efficiency of the automated Motility and Fluorescence Assay (MFA) system BallastWISE, which automatically counts motile and fluorescent organisms in the fractions ≥10 - <50 μm and ≥ 50 μm, according to the ballast water discharge standard. Standardized counting methods for comparison were completed under microscope. Organisms in the ≥10 - <50 μm size range were analysed using an FDA (Fluorescein diacetate) and CMFDA (5-chloromethylfluorescein diacetate) staining method, and a standard movement and response to stimuli technique was used for the ≥50 μm size range. BallastWISE results correlated well with direct microscopic counts, showing promising counting performance and efficiency when it comes to rapid analysis of ballast water plankton samples. Quantification of monoculture and mixed cultures using BallastWISE correlated well with manual viable counts (Tetraselmis suecica in size range ≥ 10 - <50 μm: Pearson correlation (r) = 1.0; Tigriopus californicus and Brachionus plicatilis in size range ≥ 50 μm: r = 0.99). BallastWISE counts also correlated well with viable microscopic counts of ambient organisms in natural seawater (size range ≥ 10 - <50 μm: r = 0.97 and size range ≥ 50 μm: r = 0.89). We propose that computer-assisted quantification of plankton provides a rapid and precise alternative to traditional counting and identification of organisms in water samples such as ballast water.
更新日期:2020-04-19

 

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