The circadian clock regulates the timing of many aspects of plant physiology, and this requires entrainment of the clock to the prevailing day:night cycle. Different plant cells and tissues can oscillate with different free-running periods, so coordination of timing across the plant is crucial. Previous work showed that a major difference between the clock in mature shoots and roots involves light inputs. The objective of this work was to define, in Arabidopsis thaliana, the operation of the root clock in more detail, and in particular how it responds to light quality. Luciferase imaging was used to study the shoot and root clocks in several null mutants of clock components and in lines with aberrant expression of phytochromes. Mutations in each of the components of the evening complex (EARLY FLOWERING 3 and 4, and LUX ARRHYTHMO) were found to have specific effects on roots, by affecting either rhythmicity or period and its response to light quality. The data suggest that the evening complex is a key part of the light input mechanism that differs between shoots and roots and show that roots sense red light via phytochrome B. This article is protected by copyright. All rights reserved.

中文翻译：

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晚间复合体是拟南芥枝条和根部生物钟差异的核心

生物钟调节植物生理学的许多方面的时间，这需要将生物钟夹带到主要的昼夜循环中。不同的植物细胞和组织可以以不同的自由运行期振荡，因此整个植物的时间协调至关重要。以前的工作表明，成熟芽和根的时钟之间的主要差异涉及光输入。这项工作的目的是在拟南芥中更详细地定义根时钟的运行，特别是它如何响应光质量。荧光素酶成像用于研究几个时钟组件无效突变体中的枝条和根时钟，并与光敏色素的异常表达一致。晚间综合体的每个组成部分的突变（早花 3 和 4，和 LUX ARRHYTHMO) 被发现通过影响节律或周期及其对光质量的响应对根有特定影响。数据表明晚间复合物是光输入机制的关键部分，在芽和根之间存在差异，并表明根通过光敏色素 B 感知红光。本文受版权保护。版权所有。