Survival of coral reef‐associated Symbiodiniaceae is vital to maintain the healthy coral community in coral reefs. However, knowledge about cultivation of free‐living or symbiotic Symbiodiniaceae has been limited. In this study, the response surface methodology was applied to optimize the medium for Effrenium voratum. The results showed that the impacts of nutrient components on algal growth were: FeCl₃> NaH₂PO₄>MnSO₄> MgSO₄/CoSO₄ > KCl>ZnSO₄> CaCl₂/NaNO₃, among which NaH₂PO₄ and FeCl₃ significantly affected algal growth. The optimal medium was: natural seawater supplemented with NaH₂PO₄·2H₂O 0.25 mM,FeCl₃·6H₂O 14.24 μM, NaNO₃ 0.94 mM, MgSO₄·7H₂O 40.63 mM, KCl 5.37 mM, CaCl₂·2H₂O 4.08 mM, ZnSO₄·7H₂O 0.35 μM, MnSO₄ 9.93 μM, and CoSO₄ 0.36 μM. The use of the optimized medium resulted in an increase of biomass yield (0.76 g dry weight · L⁻¹) by 46% over that using the initial medium, which agreed with the predicted value (0.71 g · L⁻¹). Additionally, fatty acids, mainly consisting of palmitic acid (C16:0) and ethyl carbonate (C20:0), accounted for approximately 50% of the total fatty acids in E. voratum. Interestingly, docosahexaenoic acid (DHA) accounted for 6% of total fatty acids, a high proportion that makes E. voratum a potential candidate feedstock in aquaculture for DHA production.

中文翻译：

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使用响应面方法优化培养基，以增强叶（Symbiodiniaceae，Dinophyceae）的生长。

与珊瑚礁相关的共生植物的生存对于维持珊瑚礁中健康的珊瑚群落至关重要。但是，关于自由生活或共生共生双生科的栽培的知识是有限的。在这项研究中，响应面方法被用于优化E叶的培养基。结果表明，营养成分对藻类生长的影响为：FeCl ₃ > NaH ₂ PO ₄ > MnSO ₄ > MgSO ₄ / CoSO ₄ > KCl> ZnSO ₄ > CaCl ₂ / NaNO ₃，其中NaH ₂ PO ₄和FeCl ₃显着影响藻类的生长。最佳培养基是：天然海水，补充NaH ₂ PO ₄ ·2H ₂ O 0.25 mM，FeCl ₃ ·6H ₂ O 14.24μM，NaNO ₃ 0.94 mM，MgSO ₄ ·7H ₂ O 40.63 mM，KCl 5.37 mM，CaCl ₂ · 2H ₂ O 4.08 mM，ZnSO ₄ ·7H ₂ O 0.35μM，MnSO ₄ 9.93μM和CoSO ₄ 0.36μM。优化培养基的使用使生物量产量（0.76 g干重·L ^-1）比初始培养基增加了46％，与预测值（0.71 g·L一致）^-1）。另外，主要由棕榈酸（C16：0）和碳酸乙酯（C20：0）组成的脂肪酸约占禾草总脂肪酸的50％。有趣的是，二十二碳六烯酸（DHA）占总脂肪酸的6％，这一比例很高，使得伏地虎成为水产养殖中生产DHA的潜在候选原料。