Silver nanoparticles (AgNPs), synthesized by green methods with the property to kill microbes, are highly valuable in medical sciences. So, the current study was aimed at using the flower extract of Wedelia urticifolia for synthesizing AgNPs with antibacterial properties. The AgNPs were produced by adding the extract to three different AgNO₃ concentrations (1, 10, and 100 mM) in nine possible flower extract to metal salt ratios (9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8, and 1:9). The formation of brown color and the presence of a peak at 431 nm in the UV–Vis spectrum of the colloidal solution indicates the synthesis of AgNPs, which were also characterized by dynamic light scattering (DLS), Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, transmission electron microscopy, and energy‐dispersive X‐ray spectroscopy. The DLS results exposed that the smallest sized AgNPs were obtained in 10 mM AgNO₃ solution and 4E6M was the optimized extract to metal salt solution ratio. The characterization techniques revealed that the synthesized AgNPs were spherical shaped and crystalline with a diameter of less than 30 nm. Furthermore, the synthesized nanoparticles were tested against two Gram‐positive (Klebsiella pneumonia and Staphylococcus aureus) and two Gram‐negative (Pseudomonas aeruginosa and Escherichia coli) bacterial strains for their antibacterial efficiency. Although the studied strains showed limited growth, overall, the effect of nanoparticles was found to be insignificant. It is concluded that the current study is advantageous over other previous studies because the AgNPs were synthesized at room temperature from 10 mM AgNO₃ concentration in only 2 hours. Additionally, the present work is simple, ecofriendly, and in‐expensive.

中文翻译：

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使用Wedelia urticifolia（Blume）DC合成银纳米颗粒。花提取物：表征和抗菌活性评估。

通过绿色方法合成的具有杀死微生物特性的银纳米颗粒（AgNPs）在医学领域具有很高的价值。因此，目前的研究旨在使用的花提取物蟛urticifolia与抗菌性能合成的AgNPs。通过将提取物添加到三种不同的AgNO _3中来生产AgNP九种可能的花提取物与金属盐的比例（9：1、8：2、7：3、6：4、5：5、4：6、3：7、2：9）中的浓度（1、10和100 mM） 8和1：9）。胶体溶液的UV-Vis光谱中棕色的形成和在431 nm处存在一个峰表明AgNP的合成，其特征还在于动态光散射（DLS），傅里叶变换红外光谱，X射线衍射，扫描电子显微镜，透射电子显微镜和能量色散X射线光谱。DLS结果表明，在10 mM AgNO ₃中获得了最小尺寸的AgNP。溶液和4E6M是提取液与金属盐溶液的最佳比例。表征技术表明，合成的AgNPs是球形的且结晶的，直径小于30nm。此外，针对两种革兰氏阳性菌（肺炎克雷伯菌和金黄色葡萄球菌）和两种革兰氏阴性菌（铜绿假单胞菌和大肠杆菌）分别测试了合成的纳米颗粒的抗菌效率。尽管所研究的菌株显示出有限的生长，但总体而言，发现纳米颗粒的作用微不足道。结论是当前的研究优于其他先前的研究，因为AgNPs是在室温下由10 mM AgNO合成的₃集中仅2小时。此外，目前的工作简单，环保且价格便宜。