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Cold-inducible RNA-binding protein Cirp, but not Rbm3, may regulate transcript processing and protection in tissues of the hibernating ground squirrel.
Cell Stress and Chaperones ( IF 3.8 ) Pub Date : 2020-04-19 , DOI: 10.1007/s12192-020-01110-3 Samantha M Logan 1 , Kenneth B Storey 1
Cell Stress and Chaperones ( IF 3.8 ) Pub Date : 2020-04-19 , DOI: 10.1007/s12192-020-01110-3 Samantha M Logan 1 , Kenneth B Storey 1
Affiliation
RNA-binding proteins (RBPs) have important roles in transcription, pre-mRNA processing/transport, mRNA degradation, translation, and non-coding RNA processing, among others. RBPs that are expressed in response to cold stress, such as Cirp and Rbm3, could regulate RNA stability and translation in hibernating mammals that reduce their body temperatures from 37 °C to as low as 0-5 °C during torpor bouts. RBPs including Cirp, Rbm3, and stress-inducible HuR translocate from the nucleus to stabilize mRNAs in the cytoplasm, and thereby could regulate which mRNA transcripts are protected from degradation and are translated, versus stored, for future protein synthesis or degraded by nucleases during cell stress associated with metabolic rate depression. This is the first study to explore the transcriptional/translational regulation, and subcellular localization of cold-inducible RBPs in a model hibernator, the 13-lined ground squirrel (Ictidomys tridecemlineatus). Cirp protein levels were upregulated in liver, skeletal muscle, and brown adipose tissue throughout the torpor-arousal cycle whereas Rbm3 protein levels stayed constant or decreased, suggesting an important role for Cirp, but likely not Rbm3, in the hibernator stress response. Increased cytoplasmic localization of Cirp in liver and muscle and HuR in liver during torpor, but no changes in the relative levels of Rbm3 in the cytoplasm, emphasizes a role for Cirp and possibly HuR in regulating mRNA processing during torpor. This study informs our understanding of the natural adaptations that extreme animals use in the face of stress, and highlight natural stress response mediators that could be used to bolster cryoprotection of human organs donated for transplant.
中文翻译:
冷诱导的 RNA 结合蛋白 Cirp,但不是 Rbm3,可以调节冬眠地松鼠组织中的转录处理和保护。
RNA 结合蛋白 (RBP) 在转录、mRNA 前体加工/运输、mRNA 降解、翻译和非编码 RNA 加工等方面具有重要作用。响应冷应激而表达的 RBP,如 Cirp 和 Rbm3,可以调节冬眠哺乳动物的 RNA 稳定性和翻译,在冬眠期间将体温从 37°C 降低到 0-5°C。包括 Cirp、Rbm3 和应激诱导型 HuR 在内的 RBP 从细胞核易位以稳定细胞质中的 mRNA,从而可以调节哪些 mRNA 转录物免受降解和翻译,而不是储存,以备将来蛋白质合成或在细胞期间被核酸酶降解与代谢率降低相关的压力。这是第一项探索转录/翻译调控的研究,冷诱导 RBP 在模型冬眠者中的亚细胞定位,13 线地松鼠(Ictidomys tridecemlineatus)。在整个昏睡-觉醒周期中,肝脏、骨骼肌和棕色脂肪组织中的 Cirp 蛋白水平上调,而 Rbm3 蛋白水平保持不变或降低,这表明 Cirp 在冬眠应激反应中发挥重要作用,但可能不是 Rbm3。麻痹期间肝脏和肌肉中 Cirp 的细胞质定位增加,肝脏中的 HuR 增加,但细胞质中 Rbm3 的相对水平没有变化,这强调了 Cirp 和可能的 HuR 在调节麻木期间 mRNA 加工中的作用。这项研究让我们了解了极端动物在面对压力时的自然适应能力,
更新日期:2020-04-20
中文翻译:
冷诱导的 RNA 结合蛋白 Cirp,但不是 Rbm3,可以调节冬眠地松鼠组织中的转录处理和保护。
RNA 结合蛋白 (RBP) 在转录、mRNA 前体加工/运输、mRNA 降解、翻译和非编码 RNA 加工等方面具有重要作用。响应冷应激而表达的 RBP,如 Cirp 和 Rbm3,可以调节冬眠哺乳动物的 RNA 稳定性和翻译,在冬眠期间将体温从 37°C 降低到 0-5°C。包括 Cirp、Rbm3 和应激诱导型 HuR 在内的 RBP 从细胞核易位以稳定细胞质中的 mRNA,从而可以调节哪些 mRNA 转录物免受降解和翻译,而不是储存,以备将来蛋白质合成或在细胞期间被核酸酶降解与代谢率降低相关的压力。这是第一项探索转录/翻译调控的研究,冷诱导 RBP 在模型冬眠者中的亚细胞定位,13 线地松鼠(Ictidomys tridecemlineatus)。在整个昏睡-觉醒周期中,肝脏、骨骼肌和棕色脂肪组织中的 Cirp 蛋白水平上调,而 Rbm3 蛋白水平保持不变或降低,这表明 Cirp 在冬眠应激反应中发挥重要作用,但可能不是 Rbm3。麻痹期间肝脏和肌肉中 Cirp 的细胞质定位增加,肝脏中的 HuR 增加,但细胞质中 Rbm3 的相对水平没有变化,这强调了 Cirp 和可能的 HuR 在调节麻木期间 mRNA 加工中的作用。这项研究让我们了解了极端动物在面对压力时的自然适应能力,