Chickpeas are consumed in various edible forms and hence presence of NPT-II proteins encoded by kanamycin resistance gene, NPT-II, in transgenic chickpea seeds raises biosafety concerns related to human health in particular. We estimated the NPT-II protein from various tissues like leaf, pod wall, immature seeds and mature dried seeds of five different transgenic chickpea lines expressing NPT-II. Quantitative ELISA studies indicated that the amount of NPT-II proteins decrease, as chickpea plants approached physiological maturity with mature seeds having lowest NPT-II content (0.98–1.32 ng/mg Total Soluble Protein). Mature chickpea seeds are most commonly used for edible purpose and the content of NPT-II estimated possess no discernible biosafety concern. Further, bioinformatics studies also indicate that NPT-II proteins are innocuous and possibly should not be active in acidic environment in the human gut.

中文翻译：

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转基因鹰嘴豆（Cicer arietinum L.）营养和生殖组织中新霉素磷酸转移酶II（NPT-II）蛋白的估计和生物安全性观点

鹰嘴豆以各种食用的形式消耗，因此通过卡那霉素抗性基因，编码NPT-II蛋白质的存在NPT -II，在转基因鹰嘴豆提高尤其关系到人体健康的生物安全的担忧。我们从表达NPT的五种不同转基因鹰嘴豆品系的各种组织（如叶片，豆荚壁，未成熟的种子和成熟的干燥种子）中估计了NPT-II蛋白-II。ELISA定量研究表明，随着鹰嘴豆植物接近生理成熟，其成熟种子的NPT-II含量最低（0.98–1.32 ng / mg总可溶性蛋白），NPT-II蛋白的含量降低。成熟的鹰嘴豆种子最常用于食用，估计的NPT-II含量没有明显的生物安全隐患。此外，生物信息学研究还表明，NPT-II蛋白是无害的，在人体肠道的酸性环境中可能不具有活性。