Halophilic Halomonas bluephagenesis (H. bluephagenesis), a chassis for cost-effective Next Generation Industrial Biotechnology (NGIB), was for the first time engineered to successfully produce L-threonine, one of the aspartic family amino acids (AFAAs). Five exogenous genes including thrA*BC, lysC* and rhtC encoding homoserine dehydrogenase mutant at G433R, homoserine kinase, L-threonine synthase, aspartokinase mutant at T344M, S345L and T352I, and export transporter of threonine, respectively, were grouped into two expression modules for transcriptional tuning on plasmid- and chromosome-based systems in H. bluephagenesis, respectively, after pathway tuning debugging. Combined with deletion of import transporter or/and L-threonine dehydrogenase encoded by sstT or/and thd, respectively, the resulting recombinant H. bluephagenesis TDHR3-42-p226 produced 7.5 g/L and 33 g/L L-threonine when grown under open unsterile conditions in shake flasks and in a 7 L bioreactor, respectively. Engineering H. bluephagenesis demonstrates strong potential for production of diverse metabolic chemicals.

嗜盐的嗜盐单胞菌（H. bluephagenesis），是经济高效的下一代工业生物技术（NGIB）的底盘，首次成功地成功生产了L-苏氨酸，这是天冬氨酸家族氨基酸（AFAAs）之一。将五个外源基因分别编码为两个表达模块，其中包括分别在G433R处编码高丝氨酸脱氢酶突变体的thrA * BC，lysC *和rhtC，高丝氨酸激酶，L-苏氨酸合酶，天冬氨酸激酶突变体（T344M，S345L和T352I）和苏氨酸的出口转运蛋白。蓝细菌中基于质粒和染色体的系统的转录调控分别经过路径优化调试后。结合缺失分别由sstT或/和thd编码的进口转运蛋白或/和L-苏氨酸脱氢酶，在室温下生长时，所得的重组H. bluephagenesis TDHR3-42-p226产生7.5 g / L和33 g / L L-苏氨酸分别在摇瓶和7 L生物反应器中打开无菌条件。工程蓝细菌证明了生产多种代谢化学物质的强大潜力。