Next generation sequencing was used to determine the whole genome sequence for two different strains of guinea pig adenovirus (GPAdV) detected in association with outbreaks of pneumonia in Australia in 1996, and in Germany in 1997 using total DNA extracted from infected archival frozen lung tissue as a template. The length of the determined genomic sequences was 37,031 bp and 37,070 bp, respectively. The nucleotide composition showed a relatively high content of guanine + cytosine (G + C) of 62 %. The 99.6 % nucleotide identity between the two sequenced viruses suggests that they may represent variants of the same genotype. The GPAdV genome exhibits the genomic features of a typical mastadenovirus with at least 32 open reading frames identified. Five novel open reading frames were found at the right end of the genomic sequence. One of them maps to the predicted E3 region and encodes a putative CR1 protein, two map to the E4 region, and two map to the l strand of L1 and L3, respectively. Our phylogenetic analysis of whole genome sequences showed that among the mammalian AdV species described to date, GPAdV is most closely related to MAdV-2 The characterization of this mastadenovirus species offers an opportunity to develop a new small animal model to study mammalian adenovirus pathogenesis.

中文翻译：

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从档案肺组织中回收的两种豚鼠腺病毒株的基因组和系统发育分析。

下一代测序用于确定与 1996 年在澳大利亚和 1997 年在德国爆发的肺炎相关的两种不同的豚鼠腺病毒 (GPAdV) 毒株的全基因组序列，使用从受感染的档案冷冻肺组织中提取的总 DNA 作为一个模板。确定的基因组序列的长度分别为 37,031 bp 和 37,070 bp。核苷酸组合物显示出相对较高的鸟嘌呤 + 胞嘧啶 (G + C) 含量，为 62%。两种测序病毒之间 99.6% 的核苷酸同一性表明它们可能代表相同基因型的变体。GPAdV 基因组表现出典型乳腺腺病毒的基因组特征，至少有 32 个已识别的开放阅读框。在基因组序列的右端发现了五个新的开放阅读框。其中一个映射到预测的 E3 区域并编码假定的 CR1 蛋白，两个映射到 E4 区域，两个映射到 L1 和 L3 的 l 链，分别。我们对全基因组序列的系统发育分析表明，在迄今为止描述的哺乳动物 AdV 物种中，GPAdV 与 MAdV-2 的关系最密切。这种乳腺腺病毒物种的特征为开发新的小动物模型以研究哺乳动物腺病毒的发病机制提供了机会。