Abstract Boron nitride nanotubes (BNNT) were functionalized under mild conditions, using a difunctional amine, such as glycine, with one of three targeting ligands, folic acid, a nerve growth factor, or an antibody against nerve growth factor. In addition, non-fouling BNNTs were obtained by a facile and versatile, non-destructive method via controlled surface-initiated grafting of polyzwitterions. The BNNTs were loaded with a fluorescent probe for convenient imaging of glioblastoma multiforme cells treated with BNNTs. BNNTs bearing targeting factors on their outer surface demonstrated an increased efficiency of internalization in glioblastoma multiforme cells, compared to non-modified BNNTs. The degree of internalization was affected both by the nature of the ligand/agonist linked to the BNNTs surface and by the presence of serum proteins. The polyzwitterion grafts prevented the spontaneous adsorption of serum proteins on the BNNTs.

中文翻译：

---

双功能化氮化硼纳米管靶向多形性胶质母细胞瘤

摘要 氮化硼纳米管 (BNNT) 在温和条件下使用双官能胺（如甘氨酸）与三种靶向配体之一、叶酸、神经生长因子或神经生长因子抗体进行功能化。此外，通过可控的表面引发的多两性离子接枝，通过一种简便、通用、无损的方法获得了无污染的 BNNT。BNNTs 装有荧光探针，便于对用 BNNTs 处理的多形性胶质母细胞瘤细胞进行成像。与未修饰的 BNNT 相比，在其外表面上带有靶向因子的 BNNT 在多形性胶质母细胞瘤细胞中表现出更高的内化效率。内化程度受连接到 BNNT 表面的配体/激动剂的性质和血清蛋白的存在的影响。