Apigenin, is a natural compound that found in high amounts in vegetables and fruits. This natural flavone has shown strong cardioprotective effects in animal and clinical studies. Due to cardioprotective effects of apigenin in previous studies, we hypothesized that apigenin protects isolated cardiomyocytes from aluminum phosphide(AlP)-induced toxicity as the most common disturbances after exposure with this agent. By using of biochemical and flowcytometry techniques; cell viability, reactive oxygen species (ROS) generation, mitochondria membrane potential (MMP), lysosomal membrane integrity, malondialdehyde (MDA) and oxidized/reduced glutathione (GSH/GSSG) content were measured in rat heart isolated cardiomyocytes. Our results showed that the administration of apigenin (5-100 μM) efficiently decreased (P < .05) cytotoxicity, oxidative, lysosomal and mitochondrial damages induced by AlP (20 μg/ml) in isolated cardiomyocytes. Taken together, apigenin protected the cardiomyocytes against AlP toxicity via the protection of mitochondria and lysosome mediated by its antioxidant properties.

中文翻译：

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芹菜素通过减少大鼠心肌细胞的线粒体/溶酶体损伤和氧化应激来减弱磷化铝诱导的细胞毒性

芹菜素是一种天然化合物，在蔬菜和水果中含量很高。这种天然黄酮在动物和临床研究中显示出强大的心脏保护作用。由于芹菜素在先前研究中的心脏保护作用，我们假设芹菜素保护分离的心肌细胞免受磷化铝 (AlP) 诱导的毒性，这是接触该药剂后最常见的干扰。通过使用生化和流式细胞术技术；在大鼠心脏分离的心肌细胞中测量细胞活力、活性氧 (ROS) 生成、线粒体膜电位 (MMP)、溶酶体膜完整性、丙二醛 (MDA) 和氧化/还原型谷胱甘肽 (GSH/GSSG) 含量。我们的结果表明，芹菜素 (5-100 μM) 的给药有效地降低了 (P < .05) 细胞毒性、氧化性、AlP (20 μg/ml) 在分离的心肌细胞中诱导溶酶体和线粒体损伤。总之，芹菜素通过其抗氧化特性介导的线粒体和溶酶体的保护来保护心肌细胞免受 AlP 毒性。