Two Marinobacter sp. NP-4 and NP-6 were isolated from a deep oceanic basaltic crust at North Pond, located at the western flank of the Mid-Atlantic Ridge. These two strains are capable of using multiple carbon sources such as acetate, succinate, glucose and sucrose while take oxygen as a primary electron acceptor. The strain NP-4 is also able to grow anaerobically under 20 MPa, with nitrate as the electron acceptor, thus represents a piezotolerant. To explore the metabolic potentials of Marinobacter sp. NP-4 and NP-6, the complete genome of NP-4 and close-to-complete genome of NP-6 were sequenced. The genome of NP-4 contains one chromosome and two plasmids with the size of 4.6 Mb in total, and with average GC content of 57.0%. The genome of NP-6 is 4.5 Mb and consists of 6 scaffolds, with an average GC content of 57.1%. Complete glycolysis, citrate cycle and aromatics compounds degradation pathways are identified in genomes of these two strains, suggesting that they possess a heterotrophic life style. Additionally, one plasmid of NP-4 contains genes for alkane degradation, phosphonate ABC transporter and cation efflux system, enabling NP-4 extra surviving abilities. In total, genomic information of these two strains provide insights into the physiological features and adaptation strategies of Marinobacter spp. in the deep oceanic crust biosphere.

两个Marinobacter sp。NP-4和NP-6是从位于北大西洋中脊西翼的北部池塘的深海玄武质地壳中分离出来的。这两个菌株能够使用多种碳源，例如乙酸盐，琥珀酸盐，葡萄糖和蔗糖，同时以氧气为主要电子受体。NP-4菌株还能够在20 MPa下厌氧生长，硝酸盐作为电子受体，因此代表了耐压电性。探索马林杆菌的代谢潜能sp。对NP-4和NP-6，NP-4的完整基因组和NP-6的接近完整的基因组进行测序。NP-4的基因组包含一个染色体和两个质粒，总大小为4.6 Mb，平均GC含量为57.0％。NP-6的基因组为4.5 Mb，由6个支架组成，平均GC含量为57.1％。在这两个菌株的基因组中确定了完整的糖酵解，柠檬酸盐循环和芳香族化合物降解途径，表明它们具有异养型生活方式。另外，NP-4的一个质粒包含烷烃降解基因，膦酸ABC转运蛋白和阳离子外排系统，从而使NP-4具有额外的生存能力。总的来说，这两个菌株的基因组信息提供了对Marinobacter的生理特征和适应策略的见解。spp。在深海地壳生物圈中。