The incidence of potentially pathogenic Vibrio species in the marine environment around Europe, is correlated with the increase of surface seawater temperature. Despite their importance, little is known about the trigger factors of potential outbreak-causing strains in this region. As prophages may compose a major reservoir of virulence traits in marine ecosystems, this study aims to identify and characterize the genomes of lysogenic Vibrio phages exemplarily from the North Sea. Therefore, 31 isolates from potentially pathogenic Vibrio species from the North Sea were screened for inducible prophages with mitomycin C. From them, one V. cholerae isolate and 40% V. parahaemolyticus isolates carried inducible prophages. Three lysogenic phages were selected for genomic characterization. The phage vB_VpaM_VP-3212 (unclassified Myoviridae) has a genome with a length of 36.81 Kbp and 55 CDS were identified. This lysogenic phage of V. parahaemolyticus contains genes related to replicative transposition mechanism, such as transposase and mobile elements similar to Mu-like viruses. The phage vB_VpaP_VP-3220 (Podoviridae, unclassified Nona33virus) has a genome length of 58,14 Kbp and contains 63 CDS. This V. parahaemolyticus phage probably uses a headful (pac) packaging replication mechanism. The phage vB_VchM_VP-3213 (unclassified Myoviridae) has a genome with a length of 41 Kbp and 63 CDS were identified, including integrase and Xer system for lysogenic recombination. This lysogenic phage of V. cholerae has similar genomic features as lambdoid phages. Although no pathogenicity genes were identified, their similarity among other phage genomes indicates that these phages can affect the development of pathogenic Vibrio strains in marine environments.

欧洲周围海洋环境中潜在致病性弧菌的发生率与地表海水温度的升高有关。尽管它们很重要，但对该地区潜在的引起疾病爆发的菌株的触发因素知之甚少。由于噬菌体可能构成海洋生态系统中主要的毒力特征库，因此本研究旨在鉴定和表征北海示例性溶源性弧菌噬菌体的基因组。因此，从丝裂霉素C中筛选了来自北海的31种来自潜在致病性弧菌的分离株，寻找诱导性原菌。从中分离出一种霍乱弧菌和40％副溶血弧菌。分离株携带可诱导的噬菌体。选择了三个溶原性噬菌体用于基因组表征。噬菌体vB_VpaM_VP-3212（未分类的肌病毒科）的基因组长度为36.81 Kbp，鉴定出55个CDS。副溶血性弧菌的溶原性噬菌体含有与复制转座机制有关的基因，例如转座酶和类似于Mu样病毒的移动元件。噬菌体vB_VpaP_VP-3220（Podoviridae，未分类的Nona33病毒）的基因组长度为58,14 Kbp，包含63个CDS。该副溶血性弧菌噬菌体可能使用无头（pac）包装复制机制。噬菌体vB_VchM_VP-3213（未分类的肌病毒科）具有一个长度为41 Kbp的基因组，已鉴定出63个CDS，包括整合酶和用于溶原重组的Xer系统。霍乱弧菌的溶原性噬菌体具有与类λ噬菌体相似的基因组特征。尽管未鉴定出致病性基因，但它们与其他噬菌体基因组之间的相似性表明，这些噬菌体可影响海洋环境中致病性弧菌菌株的发育。