The tumor suppressor protein, p53 plays a crucial role in protecting genetic integrity. Once activated by diverse cell stresses, p53 reversibly activates downstream target genes to regulate cell cycle and apoptosis. However, few studies have investigated the effects of thermal stress in turbot, specifically the p53 signaling pathway. In this study, the rapid amplification of cDNA ends was used to obtain a full-length cDNA of the turbot p53 gene (Sm-p53) and perform bioinformatics analysis. The results showed that the cDNA of the Sm-p53 gene was 2928 bp in length, encoded a 381 amino acid protein, with a theoretical isoelectric point of 6.73. It was composed of a DNA binding and a tetramerization domain. Expression of Sm-p53 in different tissues was detected and quantified by qRT-PCR, and was highest in the liver. We also investigated the expression profiles of Sm-p53 in different tissue and TK cells after thermal stress. These result suggested that Sm-p53 plays a key role, and provides a theoretical basis for Sm-p53 changes in environmental stress responses in the turbot.

中文翻译：

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大菱鲆p53的分子克隆、表征和表达分析及其对热应激的反应

肿瘤抑制蛋白 p53 在保护遗传完整性方面起着至关重要的作用。一旦被不同的细胞应激激活，p53 就会可逆地激活下游靶基因以调节细胞周期和细胞凋亡。然而，很少有研究调查热应激对大菱鲆的影响，特别是 p53 信号通路。本研究利用cDNA末端的快速扩增获得大菱鲆p53基因(Sm-p53)的全长cDNA并进行生物信息学分析。结果表明，Sm-p53基因的cDNA全长2928 bp，编码381个氨基酸的蛋白质，理论等电点为6.73。它由一个 DNA 结合域和一个四聚化域组成。通过qRT-PCR检测和定量Sm-p53在不同组织中的表达，在肝脏中表达最高。我们还研究了热应激后 Sm-p53 在不同组织和 TK 细胞中的表达谱。这些结果表明 Sm-p53 起关键作用，为 Sm-p53 改变大菱鲆环境应激反应提供了理论依据。