Abstract Lipoxygenases (LOXs, EC 1.13.11.12), which effected the quality of soybean, rice and maize in storage processing, is the key enzyme in seed lipid metabolism ways, but is rarely reported in peanut. In order to determine the activity of LOXs in peanut, a quantitative method for the determination of LOX3 activity in peanut was developed, which was optimized on the basis of the method of Lox3 Beta-carotene coupling colorimetry. The optimum sample size was 8 mg, and the optimum time was 10–70s after reaction. The distribution of LOX3 activity of 214 peanut germplasm resources was studied by this method, which showed that the difference of enzyme activity between the materials was very significant P

中文翻译：

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脂氧合酶3对花生种子贮藏特性的影响

摘要 脂肪氧化酶（LOXs，EC 1.13.11.12）在贮藏加工过程中影响大豆、水稻和玉米的品质，是种子脂质代谢途径中的关键酶，但在花生中报道较少。为测定花生中LOXs的活性，在Lox3β-胡萝卜素偶联比色法的基础上进行优化，建立了花生中LOX3活性的定量测定方法。最佳样品量为8 mg，最佳反应时间为反应后10-70s。采用该方法对214份花生种质资源的LOX3活性分布进行了研究，表明材料间酶活性差异非常显着P