The main aim of the study is to develop and validate a simple and rapid liquid chromatographic analytical method for simultaneous determination of trace level of un‐sulfated alcohol impurities in sodium lauryl sulfate using high‐performance liquid chromatography with a refractive index detector. The chromatographic separation was achieved using flow rate of 3.0 ml/min with a Waters Symmetry C₁₈ (150 × 4.6 mm, 5 μm) column and 50°C as a column temperature. The mobile phase comprised milliQ water and acetonitrile in the ratio of 30:70 v/v respectively. The detection was performed using a refractive index detector at a sensitivity of 64. The resolutions among n‐ decanol, n‐ dodecanol, n‐ tetradecanol, n‐ hexadecanol, and n‐ heptadecanol were found to be >4. Regression analysis showed a correlation coefficient for the stated compounds of >0.999. The validated HPLC method was utilized effectively for the estimation of un‐ sulfated alcohols in quality control labs for commercial release of sodium lauryl sulfate.

中文翻译：

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新型RP-HPLC折射率检测器方法的开发和验证，用于测定十二烷基硫酸钠原料中的痕量醇（未硫酸化）。

该研究的主要目的是开发和验证一种简单而快速的液相色谱分析方法，该方法可以使用带有折射率检测器的高效液相色谱法同时测定月桂基硫酸钠中痕量的未硫酸化乙醇杂质。色谱分离是通过使用3.0 ml / min的流速，Waters Symmetry C ₁₈（150×4.6 mm，5μm）色谱柱和50°C作为色谱柱温度实现的。流动相分别以30:70 v / v的比例包含milliQ水和乙腈。使用折射率检测器，在64的灵敏度进行检测之间的分辨率正癸醇，正十二烷醇，正十四烷醇，正发现十六烷醇和正十七烷醇> 4。回归分析显示所述化合物的相关系数> 0.999。经验证的HPLC方法对于UN的估计有效地利用-硫酸化醇在质量控制实验室为月桂基硫酸钠的商业发行。