Ophiocordyceps xuefengensis (O. xuefengensis) , a new species of caterpillar fungus, has been identified as the sister taxon of Ophiocordyceps sinensis (O. sinensis). The aims of the present study are to evaluate the anticancer activity and to qualitatively analyze the potential bioactive chemical constituents of O. xuefengensis and O. sinensis , comparatively. An MTT assay was used to evaluate the in vitro anticancer activities of different fractions from O. xuefengensis and O. sinensis . The results show that ethyl acetate fractions of O. xuefengensis and O. sinensis have significant in vitro anticancer activity. These two bioactive fractions were analyzed by ultra‐performance liquid chromatography–electrospray ionization with quadrupole–time of flight tandem mass spectrometry technology. A total of 82 compounds and 101 compounds were identified or tentatively characterized in the bioactive fractions of O. xuefengensis and O. sinensis , respectively. Among these compounds, 68 existed in both O. xuefengensis and O. sinensis . A total of 67 compounds were reported in O. xuefengensis and 8 compounds were reported in caterpillar fungus for the first time. This is the first detailed comparative analysis of the in vitro anticancer activity and chemical ingredients between O. xuefengensis and O. sinensis . The application of this work will provide reliable fundamental pharmacological substances for the use of O. xuefengensis by Yao people.

中文翻译：

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UPLC-ESI-Q-TOF-MS / MS分析雪峰麦冬和中华麦冬的抗癌成分。

冬虫夏草（O. xuefengensis）是一种新的毛虫真菌，已被确定为中华冬虫夏草（Ophiocordyceps sinensis）的姊妹分类群。本研究的目的是评估抗癌活性，并定性地分析雪枫和中华绒螯蟹的潜在生物活性化学成分。采用MTT法评价了枫香和香。不同部位的体外抗癌活性。结果表明，雪枫和中华绒螯蟹的乙酸乙酯级分在体外具有显着性。抗癌活性。这两种生物活性成分通过超高效液相色谱-四极电喷雾电离-飞行时间串联质谱技术进行了分析。分别在雪枫木和中华稻草的生物活性级分中鉴定或初步鉴定了82种化合物和101种化合物。在这些化合物中，雪枫和中华稻中均存在68种。首次在雪枫中报告了67种化合物，在毛虫真菌中首次报告了8种化合物。这是体外的第一个详细比较分析雪松和中华绒螯蟹的抗癌活性及其化学成分。这项工作的应用将为瑶族人使用雪枫提供可靠的基础药理物质。