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Identification of prototypes from Ligustri Lucidi Fructus in rat plasma based on a data-dependent acquisition and multicomponent pharmacokinetic study.
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-03-20 , DOI: 10.1002/bmc.4833
Yucheng Wang 1 , Keyu Feng 1 , Mengrong Li 1 , Lifeng Han 1 , Weiqiang Wang 1 , Dandan Si 2 , Xiaopeng Chen 1 , Wenzhi Yang 1 , Xiumei Gao 1 , Erwei Liu 1
Affiliation  

The identification and quantization of traditional Chinese medicine (TCM) are a challenge for researchers and industry. Using untargeted analytical methods, the in vivo detection and identification of TCM compounds are difficult because of the significant interference of endogenous substances. Fortunately, the ongoing development of new analytical technologies, especially Q‐Orbitrap–MS, offers some solutions. Our team developed a holistic MS method, combining untargeted data‐dependent MS2 (dd‐MS2) modes to extensively identify TCM prototypes in vivo. The method was successfully applied to the analysis of Ligustri Lucidi Fructus (LLF). LLF is a widely used TCM with a remarkable nourishing effect on the liver and kidney. In the study, we aimed to identify the prototypes in rat plasma after oral administration of LLF extract. Following separation on an HSS T3 column, LLF extract and rat plasma were performed in untargeted dd‐MS2 mode. Forty‐seven compounds were characterized in rats plasma as prototypes of LLF extract. Furthermore, seven major prototypes were chosen as pharmacokinetic markers to investigate LLF's pharmacokinetic properties. The results provides comprehensive determination of compounds in LLF both in vitro and in vivo , which is important for quality control, pharmacology studies and clinical use of LLF.

中文翻译:

基于数据依赖的采集和多组分药代动力学研究,从大鼠血浆中鉴定了Ligustri Lucidi Fructus的原型。

中药(TCM)的鉴定和量化对研究人员和工业界都是一个挑战。使用非靶向分析方法,由于内源性物质的显着干扰,很难在体内检测和鉴定中药化合物。幸运的是,新分析技术(特别是Q-Orbitrap-MS)的不断开发提供了一些解决方案。我们的团队开发了一种整体MS方法,结合了非目标数据依赖的MS 2(dd-MS 2)模式来在体内广泛鉴定TCM原型该方法已成功地应用到了金缕梅(LLF)的分析中。LLF是一种广泛使用的中药,对肝脏和肾脏具有显着的营养作用。在这项研究中,我们旨在鉴定口服LLF提取物后在大鼠血浆中的原型。在HSS T 3色谱柱上分离后,以非靶向dd-MS 2模式进行LLF提取物和大鼠血浆。在大鼠血浆中有47种化合物被表征为LLF提取物的原型。此外,选择了七个主要的原型作为药代动力学标记,以研究LLF的药代动力学特性。结果提供了体外体内LLF中化合物的全面测定,这对于LLF的质量控制,药理研究和临床使用非常重要。
更新日期:2020-03-20
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