Abstract

Cancer includes a range of diseases that arise as a result of the unregulated growth of malignant cells, which have the potential to invade or spread to other body parts. Nanoparticles and drug conjugate systems enable unique approaches for cancer treatment. The present study focused on anti-breast cancer activity of rutin-chitosan nanoconjugates in in vitro system. Rutin-chitosan nanoconjugates were prepared by ionic gelation process and characterized by UV–Vis Spectra analysis, DLS, SEM, Zeta potential, FT-IR and XRD analysis. Further the nanoconjugates was evaluated for its anti-cancer activity on Triple Negative Breast Cancer (TNBC), it shows significant activity against selected breast cancer cells. The Inhibitory concentration (IC₅₀) of synthesized nano-conjugate was found to be 12.5 µg/mL. Furthermore, we found noteworthy cell cycle arrest at DNA synthetic phase following apoptotic cell deaths which were evidenced by AO/EtBr and DAPI fluorescence micrographic analysis. Our findings apparently validate that synthesized nano-conjugates could efficiently initiate the apoptotic process in the triple negative breast cancer cells. Through this finding, it is evidenced that the synthesized nanoconjugates potential applications in anti-cancer therapies.

中文翻译：

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芦丁-壳聚糖纳米复合物在三阴性乳腺癌细胞中的抗癌和凋亡诱导作用。

摘要

癌症包括由于恶性细胞生长不受控制而引起的一系列疾病，这些疾病可能会侵入或扩散到其他身体部位。纳米颗粒和药物结合系统为癌症治疗提供了独特的方法。本研究集中于芦丁-壳聚糖纳米复合物在体外系统中的抗乳腺癌活性。芦丁-壳聚糖纳米复合物是通过离子凝胶法制备的，并通过紫外-可见光谱，DLS，SEM，Zeta电位，FT-IR和XRD分析表征。进一步评估了纳米缀合物对三阴性乳腺癌（TNBC）的抗癌活性，它显示出对选定的乳腺癌细胞的显着活性。抑制浓度（IC ₅₀合成纳米共轭物的）为12.5μg/ mL。此外，我们发现凋亡细胞死亡后在DNA合成期有明显的细胞周期停滞，这通过AO / EtBr和DAPI荧光显微照片分析得到了证明。我们的发现显然证实了合成的纳米共轭物可以有效地启动三阴性乳腺癌细胞的凋亡过程。通过该发现，证明了合成的纳米缀合物在抗癌疗法中的潜在应用。