Abstract.

Three recently observed facts of the translocation of actual hexameric and nonstructural (NS) helicases are related to the various physical quantities and are in accordance with the recently proposed mechanical mechanism: a) the translocation of hexameric helicases might be led by either the N-terminal domain (NTD) or C-terminal domain (CTD) depending on which domain has a smaller central pore, b) the translocation speed (v_t) of the ring-shaped helicases and NS helicases decreased with decreasing applied tension, and c) a large difference in the v_t of the NS helicase was observed for the helicase translocating on DNA and RNA. These findings are the effects of the physical quantities of the helicase/nuclei acid strands on the translocation of helicases and are difficult to explain with biochemical models. We predict that a similar behavior as described in b) and c) is also shown by hexameric helicases. The validity of the mechanical mechanism is demonstrated in simulation experiments.

Graphical abstract

中文翻译：

---

六聚和非结构解旋酶易位的机械机制：取决于物理参数。

摘要。

最近观察到的实际六聚体和非结构（NS）解旋酶易位的三个事实与各种物理量有关，并且符合最近提出的机械机理：a）六聚体解旋酶的易位可能由N端引起结构域（NTD）或C末端结构域（CTD），具体取决于哪个结构域具有较小的中心孔; b）环状解旋酶和NS解旋酶的转运速度（v _t）随着施加张力的降低而降低，并且c）a v _t的大差异观察到NS解旋酶的α-解旋酶在DNA和RNA上易位。这些发现是解旋酶/核酸链的物理量对解旋酶易位的影响，并且难以用生化模型来解释。我们预测六聚解旋酶也显示出与b）和c）中所述类似的行为。仿真实验证明了机械机构的有效性。

图形概要