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The Immunogenicity of a Novel Chimeric Hemagglutinin-Neuraminidase-Fusion Antigen from Newcastle Disease Virus by Oral Delivery of Transgenic Canola Seeds to Chickens.
Molecular Biotechnology ( IF 2.6 ) Pub Date : 2020-04-03 , DOI: 10.1007/s12033-020-00254-y
Mohammad Javad Motamedi 1 , Mohammad Majid Ebrahimi 2 , Shahla Shahsavandi 2 , Jafar Amani 3 , Rouhollah Kazemi 1 , Mahyat Jafari 1 , Ali-Hatef Salmanian 1
Affiliation  

Abstract

Newcastle disease (ND) is considered as one of the most devastating infectious diseases targeting domestic birds and has considerable threat to the commercial poultry production. Two surface glycoproteins, hemagglutinin-neuraminidase (HN) and fusion (F), act as antigens in the virus structure and also play important roles in infecting host cells. In the current study, the expression of the chimeric HN-F protein in canola seeds and its immunogenicity in chickens were investigated. The HN-F gene was cloned downstream of the fatty acid elongase 1 (FAE1) promoter in the binary expression vector, pBI1400-HN-F, and introduced into rapeseed (Brassica napus L.) using Agrobacterium-mediated transformation. The amount of the HN-F glycoprotein was estimated up to 0.18% and 0.11% of the total soluble protein (TSP) in transgenic seeds and leaves of canola, respectively. Confirmatory analyses of 36 transgenic lines revealed that the HN-F gene was integrated into the genome. Subsequently, HN-F protein could be expressed and accumulated in the seed tissue. Specific pathogen-free (SPF) chickens immunized orally with recombinant HN-F showed a significant rise in specific and hemagglutination inhibition (HI) antibodies 35 to 42 days post the first administration. The results implied the potential of transgenic canola seed-based expression for oral delivery of NDV immunogenic glycoproteins.



中文翻译:

新型嵌合的血凝素-神经氨酸酶融合抗原从新城疫病毒的免疫原性,通过将转基因双低油菜籽种子经口递送给鸡而产生。

摘要

新城疫(ND)被认为是针对家禽的最具破坏性的传染病之一,对商业家禽生产具有相当大的威胁。两种表面糖蛋白,血凝素神经氨酸酶(HN)和融合蛋白(F),在病毒结构中充当抗原,并且在感染宿主细胞中也起重要作用。在当前的研究中,研究了嵌合的HN-F蛋白在油菜籽中的表达及其在鸡中的免疫原性。的HN-F基因被克隆的脂肪酸在二元表达载体,pBI1400-HN-F延伸酶1(FAE1)启动子的下游,并导入油菜籽(甘蓝型油菜使用L.)农杆菌介导的转化。据估计,在双低油菜籽的转基因种子和叶片中,HN-F糖蛋白的含量分别高达总可溶性蛋白(TSP)的0.18%和0.11%。对36个转基因品系的确证分析表明,HN-F基因已整合到基因组中。随后,HN-F蛋白可以在种子组织中表达和积累。首次注射后35到42天,用重组HN-F口服免疫的无特定病原体(SPF)的鸡表现出特异性和血凝抑制(HI)抗体的显着增加。结果暗示了基于转基因低芥酸菜子种子的表达对于NDV免疫原性糖蛋白的口服递送的潜力。

更新日期:2020-04-14
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