Since the management of infections becomes prior global healthcare issue, the “post antibiotic era” requires innovative and interdisciplinary approach. As an alternative to widespread and, nowdays mostly uneffective, antibiotic treatment of infections, the series of hydrogels were developed and further investigated as novel antibacterial biomaterials. The hydrogels based on 2-hydroxyethyl acrylate and itaconic acid were synthesized and used for silver(I) ions incorporation. The structural, thermal and swelling characteristics were examined by Fourier transform infrared spectroscopy, differential scanning calorimetry, and swelling study conducted in wide range of pHs at 37 °C. Results confirmed the expected structure, while the glass transition temperatures (T_g) of the hydrogels were detected in range of 10–37 °C. The in vitro release study revealed suitability of these pH sensitive hydrogels as the systems for topical delivery of silver(I) ions. Performed MTT test and Comet assay proved biocompatibility of the hydrogels, as well as the absence of acute genotoxic effect on human fibroblast cells (MRC-5). The hydrogels exhibited satisfying antibacterial activity against methicillin sensitive Staphylococcus aureus (MSSA) and methicillin resistant Staphylococcus aureus (MRSA), indicating the capacity to treat the life-threatening infections.

中文翻译：

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pH敏感的银（I）/聚（丙烯酸2-羟乙酯/衣康酸）水凝胶的抗菌活性

由于感染的管理已成为全球医疗保健的先决问题，因此“后抗生素时代”需要创新和跨学科的方法。作为对感染的抗生素治疗的广泛替代和替代，如今这种抗生素大多无效，因此开发了一系列水凝胶并作为新型抗菌生物材料进行了进一步研究。合成了基于丙烯酸2-羟乙酯和衣康酸的水凝胶，并将其用于掺入银（I）离子。通过傅立叶变换红外光谱，差示扫描量热法和在37°C的宽pH范围内进行的溶胀研究，研究了结构，热和溶胀特性。结果证实了预期的结构，而玻璃化转变温度（T _g的水凝胶在10–37°C的温度范围内检测到。的体外释放的研究显示这些pH敏感性水凝胶为用于局部递送的银（I）离子的系统的适用性。进行的MTT试验和Comet试验证明了水凝胶的生物相容性，以及对人成纤维细胞（MRC-5）没有急性遗传毒性作用。水凝胶对甲氧西林敏感的金黄色葡萄球菌（MSSA）和耐甲氧西林的金黄色葡萄球菌（MRSA）表现出令人满意的抗菌活性，表明其具有治疗威胁生命的感染的能力。