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Identification of phytoplasma in six fruit crops in India
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2020-02-08 , DOI: 10.1007/s10658-020-01949-3
Govind Pratap Rao , Ashutosh Rao , Manish Kumar , Hemavati Ranebennur , Surabhi Mitra , Ashok Kumar Singh

Symptoms of decline, leaf yellowing and reddening, little leaf and malformation were observed in apricot, guava, lychee, mango, pomegranate and grapevine in fruit orchards belonging to three states of India namely Delhi, Maharashtra and Jammu & Kashmir during 2016–2017. Phytoplasmas belonging to three different groups were detected in all the symptomatic fruit tree samples in polymerase chain reaction with phytoplasma specific primer pairs amplifying 16S rRNA and secA genes. Pair wise sequence comparison and phylogenetic analysis confirmed the presence of phytoplasmas. A ‘Candidatus Phytoplasma asteris’ - related strain was detected in apricot showing decline symptoms in Siot (Jammu & Kashmir). ‘Ca. P. australasia’ - related strain was identified in guava, lychee, mango and pomegranate in Rajbag, Sunjwan (Jammu & Kashmir), Pusa (Delhi) and Baramati (Maharashtra), respectively, exhibiting little leaf, leaf yellows and malformation symptoms. Furthermore, a rice yellow dwarf group (16SrXI-B) - related strain was detected in pomegranate and grapevine that showed leaf yellowing and reddening at Pusa (Delhi) and Baramati (Maharashtra), respectively. Subgroup analysis using virtual RFLP of 16S rDNA sequences allowed enclosing these phytoplasma strains into 16SrI-B, 16SrII-D and 16SrXI-B subgroups. In the study, identification of phytoplasma subgroups, 16SrI-B in apricot, 16SrII-D in guava, lychee, mango and pomegranate (Pusa) and 16SrXI-B in pomegranate (Baramati) and grapevine, are the first records globally.



中文翻译:

印度六种水果作物的植原体鉴定

在2016-2017年期间,在印度三个州(德里,马哈拉施特拉邦和查mu和克什米尔邦)的果园中,杏,番石榴,荔枝,芒果,石榴和葡萄中出现了下降,叶片变黄和变红,很少叶片和畸形的症状。在所有有症状果树样品中,利用植物浆体特异性引物对扩增16S rRNA和sec A基因,在所有有症状果树样品中检测到属于三个不同种类的植物质体。配对序列比较和系统发育分析证实了植物原浆的存在。A“暂定植原体阿斯特里斯” -杏表示Siot(查漠&克什米尔)下降的症状中检测相关的应变。“。在Rajbag,Sunjwan(Jammu&Kashmir),Pusa(Delhi)和Baramati(Maharashtra)的番石榴,荔枝,芒果和石榴中分别鉴定出与澳大利亚假单胞菌相关的菌株,显示出很少的叶片,叶片黄变和畸形症状。此外,在石榴和葡萄中检测到稻黄矮化群(16SrXI-B)相关菌株,分别在普萨(德里)和巴拉马蒂(马哈拉施特拉邦)显示叶片变黄和变红。使用16S rDNA序列的虚拟RFLP进行亚组分析,可以将这些植物质原体菌株分为16SrI-B,16SrII-D和16SrXI-B亚组。在这项研究中,对植物质群亚群的鉴定:杏中的16SrI-B,番石榴,荔枝,芒果和石榴(Pusa)中的16SrII-D,石榴(Baramati)和葡萄中的16SrXI-B是全球最早的记录。

更新日期:2020-04-18
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