The detection of acyl homoserine lactones (AHLs) can render their use as biomarkers and it may provide a chance to fight against food contamination and bacterial pathogens. For this purpose, in this study, an electrochemical biosensor was designed for the detection of AHLs to prevent harmful bacterial activities. The electrochemical biosensor was constructed by coating of zinc phthalocyanine bearing stabile TEMPO radical groups (TEMPO-ZnPc) on a glassy carbon electrode (GCE) and it was utilized for AHL detection. GCE/TEMPO–ZnPc electrode acted as amperometric biosensor for 3-oxo-C₁₂-HSL, C₁₀-HSL, C₈-HSL, C₆-HSL, and C₄-HSL molecules, the sensor electrode only selectively sensed 3-oxo-C₁₂-HSL molecule among the tested AHL molecules. The sensing measurements showed that 3-oxo-C₁₂-HSL produced by Pseudomonas aeruginosa was detected between 2.32 × 10^–6 and 39.9 × 10^–6 mol dm⁻³ concentrations with 1.8 × 10^–6 mol dm⁻³ limit of detection (LOD) in water. Additionally, the electrochemical biosensor was successfully applied for the detection of AHLs in milk samples. The sensing results indicated that GCE/TEMPO–ZnPc electrode can be used as rapid, sensitive, and selective biosensor for the detection of foodborne pathogens.

中文翻译：

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检测牛奶和水中细菌污染的另一种策略：新设计的电化学生物传感器

酰基高丝氨酸内酯（AHL）的检测可以使其用作生物标记物，并且可以为抵抗食品污染和细菌病原体提供机会。为此，在本研究中，设计了一种电化学生物传感器来检测AHL，以防止有害的细菌活动。电化学生物传感器是通过在玻璃碳电极（GCE）上涂覆带有稳定TEMPO自由基基团的锌酞菁（TEMPO-ZnPc）构成的，并用于AHL检测。GCE / TEMPO–ZnPc电极充当3-oxo-C ₁₂ -HSL，C ₁₀ -HSL，C ₈ -HSL，C ₆ -HSL和C ₄ -HSL分子的安培生物传感器，传感器电极仅选择性地感应3-氧代C ₁₂被测AHL分子中的-HSL分子。感测结果表明，铜绿假单胞菌产生的3-oxo-C ₁₂ -HSL在2.32×10 ^–6至39.9×10 ^–6  mol dm ^-3浓度之间被检测到，检测限为1.8×10 ^–6  mol dm ^-3（ LOD）在水中。此外，电化学生物传感器已成功应用于牛奶样品中AHL的检测。传感结果表明，GCE / TEMPO–ZnPc电极可以用作检测食源性病原体的快速，灵敏和选择性的生物传感器。