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Decreased ascorbic acid biosynthesis in response to PMSG in the pre-pubertal female rat ovary.
Research in Veterinary Science ( IF 2.4 ) Pub Date : 2020-04-04 , DOI: 10.1016/j.rvsc.2020.03.028
Md Summon Hossain 1 , Roshan Kumar Dutta 1 , Kambadur Muralidhar 2 , Rinkoo Devi Gupta 1
Affiliation  

Ascorbic acid (AA) is known to be an important antioxidant serving as a cofactor in collagen synthesis, and thus facilitates follicular growth in the ovary. Many studies have shown that AA is synthesized in the liver and transported to other organs including ovary, however, there is no direct evidence of ascorbic acid synthesis in the ovary. Hence, we examined the expression pattern of different proteins (SMP30/GNL and GULO) involved in the AA synthesis in pre-pubertal rat, which showed significant expression of these proteins, suggesting the synthesis of AA in the ovary. Accumulation of AA in the ovary during follicular growth has been well demonstrated. However, the effect of Pregnant Mare Serum Gonadotropin (PMSG) on the AA synthesis in the ovary has not been studied in detail. Hence to decipher the effect, different doses of PMSG were injected subcutaneously into the pre-pubertal female rats, and ovarian AA level was measured after 48 h. A significant increase in AA content was observed in PMSG treated animal groups. Further, to understand the mechanism underlying ovarian AA accumulation, the expression levels of SMP30/GNL and GULO genes were measured. Expression of both the genes was significantly suppressed, which suggested a lowered AA synthesis in the PMSG treated rat ovary. For further understanding, mRNA expression of AA transporters SVCT1 and SVCT2 encoded by SLC23A1 and SLC23A2 genes respectively were measured, which showed increased level of SVCT1 expression. These observations suggested that the increased AA content might not be due to increased synthesis of AA within the ovary but possibly due to increased uptake from blood during the stimulation of follicular growth.

中文翻译:

青春期前雌性大鼠卵巢中抗PMSG的抗坏血酸生物合成减少。

已知抗坏血酸(AA)是重要的抗氧化剂,可作为胶原合成中的辅助因子,因此可促进卵巢中的卵泡生长。许多研究表明,AA是在肝脏中合成的,并运输到包括卵巢在内的其他器官,但是,没有直接证据表明抗坏血酸在卵巢中合成。因此,我们检查了青春期前大鼠AA合成中涉及的不同蛋白质(SMP30 / GNL和GULO)的表达模式,这些蛋白质显示了这些蛋白质的显着表达,表明AA在卵巢中合成。卵泡生长过程中卵巢中AA的积累已得到充分证明。但是,尚未详细研究孕妇母马血清促性腺激素(PMSG)对卵巢中AA合成的影响。因此,要破译效果,将不同剂量的PMSG皮下注射到青春期前的雌性大鼠中,并在48小时后测量卵巢AA水平。在PMSG治疗的动物组中,AA含量显着增加。此外,为了了解卵巢AA积累的潜在机制,测量了SMP30 / GNL和GULO基因的表达水平。两种基因的表达均被显着抑制,这表明PMSG处理的大鼠卵巢中AA合成降低。为了进一步理解,测定了分别由SLC23A1和SLC23A2基因编码的AA转运蛋白SVCT1和SVCT2的mRNA表达,这表明SVCT1表达水平升高。
更新日期:2020-04-14
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