Porcine epidemic diarrhea virus (PEDV) causes very high mortality in newborn piglets. The mucosal immune system in the gut must eliminate potential pathogens while maintaining a mutually beneficial relationship with the commensal microbiota. Antibodies derived from the secretory immunoglobulin A (SIgA) class, act as the first line of antigen-specific immunity in the gut by recognizing both pathogens and commensals. Therefore, the measurement of SIgA levels is an important index in evaluating PEDV infections and immune status. A simple and rapid method for the detection of PEDV-specific SIgA using an immunochromatographic test strip has been developed; incorporating a colloidal gold-labeled anti-SIgA secretory component (SC) mAb probe for the detection of anti-PEDV-specific SIgA in swine. On the strip, a gold-labeled anti-SIgA SC mAb was applied to a conjugate pad; purified PEDV particles and goat anti-mouse antibodies were blotted onto a nitrocellulose membrane to form the test and control lines, respectively. Results showed that the immunochromatographic test strip had high sensitivity and specificity. When compared with enzyme-linked immunosorbent assay, kappa value suggesting that the strip could be used to detect PEDV specific SIgA in colostrum samples. Furthermore, the strip assay is rapid and easy to perform with no requirement for professional-level skills or equipment. We found that the immunochromatographic test strip was a rapid, sensitive, and reliable method for the identification of PEDV specific SIgA, indicating its suitability for epidemiological surveillance as well as vaccine immunity when studying PEDV.

猪流行性腹泻病毒（PEDV）在新生仔猪中导致很高的死亡率。肠道中的粘膜免疫系统必须消除潜在的病原体，同时保持与共生菌群的互利关系。分泌型免疫球蛋白A（SIgA）类衍生的抗体通过识别病原体和共生体，作为肠道抗原特异性免疫的第一线。因此，SIgA水平的测量是评估PEDV感染和免疫状态的重要指标。已经开发了一种使用免疫色谱试纸条检测PEDV特异性SIgA的简单快速方法。整合了胶体金标记的抗SIgA分泌成分（SC）mAb探针，用于检测猪中的抗PEDV特异性SIgA。在地带上 将金标记的抗SIgA SC mAb应用于偶联物垫；将纯化的PEDV颗粒和山羊抗小鼠抗体印迹到硝酸纤维素膜上，分别形成测试线和对照线。结果表明，该免疫层析试纸具有较高的灵敏度和特异性。与酶联免疫吸附测定法相比，kappa值表明该试纸条可用于检测初乳样品中的PEDV特异性SIgA。此外，试纸条分析快速且易于执行，不需要专业水平的技能或设备。我们发现免疫色谱试纸是一种快速，灵敏和可靠的鉴定PEDV特异性SIgA的方法，表明它在研究PEDV时适用于流行病学监测以及疫苗免疫。将纯化的PEDV颗粒和山羊抗小鼠抗体印迹到硝酸纤维素膜上，分别形成测试线和对照线。结果表明，该免疫层析试纸具有较高的灵敏度和特异性。与酶联免疫吸附测定法相比，kappa值表明该试纸条可用于检测初乳样品中的PEDV特异性SIgA。此外，试纸条分析快速且易于执行，不需要专业水平的技能或设备。我们发现免疫色谱试纸是一种快速，灵敏和可靠的方法，用于鉴定PEDV特异性SIgA，表明其在研究PEDV时适合流行病学监测以及疫苗免疫。将纯化的PEDV颗粒和山羊抗小鼠抗体印迹到硝酸纤维素膜上，分别形成测试线和对照线。结果表明，该免疫层析试纸具有较高的灵敏度和特异性。与酶联免疫吸附测定法相比，kappa值表明该试纸条可用于检测初乳样品中的PEDV特异性SIgA。此外，试纸条分析快速且易于执行，不需要专业水平的技能或设备。我们发现免疫色谱试纸是一种快速，灵敏和可靠的方法，用于鉴定PEDV特异性SIgA，表明其在研究PEDV时适合流行病学监测以及疫苗免疫。