Canine Distemper Virus (CDV) is a highly contagious pathogen of dogs that causes severe respiratory, gastrointestinal and nervous signs. Although vaccines have been used to prevent infections, CDV has been reported worldwide, even in vaccinated animals. In the present study, a representative wild type CDV strain (Arg24) was isolated from a sick vaccinated dog and its genome was completely sequenced using Illumina technology. This strain produced a strong cytopathic effect in Vero SLAM (Signaling Lymphocyte Activation Molecule) cells with a higher titer of 1.1 × 105 Median Tissue Culture Infectious Dose (TCID50/ml) at 32 hours post infection, in cell-associated virus. The Arg24 strain genome, showed values of 97.1, 90.3, 96.7, 90.6, 89.8 and 97.3% of amino acid identity with respect to the Onderstepoort vaccine strain (Nucleoprotein, Phosphoprotein, Matrix, Fusion, Hemagglutinin and Large polymerase, respectively). Focusing on the Hemagglutinin gene, which is the target for genetic characterization, Arg24 showed four additional potential glycosylation sites, with respect to the Onderstepoort. The availability of Arg24 strain, which can be easily grown in Vero SLAM cells, is an important tool to perform immunological and antigenic comparative studies, between wild type and vaccine CDV strains.

中文翻译：

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南美2进化枝的代表犬瘟热病毒野生型菌株的病毒分离和全长基因组测序。

犬瘟热病毒（CDV）是犬的一种高度传染性病原体，可引起严重的呼吸道，胃肠道和神经系统症状。尽管已使用疫苗预防感染，但CDV已在世界范围内被报道，即使在接种疫苗的动物中也是如此。在本研究中，从有病的疫苗接种的狗中分离出代表性的野生型CDV株（Arg24），并使用Illumina技术对其基因组进行了完整测序。在感染后32小时，该菌株在Vero SLAM（信号淋巴细胞活化分子）细胞中产生了强烈的细胞病变作用，在与细胞相关的病毒中，滴度为1.1×105的组织培养感染剂量（TCID50 / ml）更高。相对于Onderstepoort疫苗株（核酸蛋白，磷酸蛋白，基质，融合蛋白，血凝素和大型聚合酶）。专注于血凝素基因，这是遗传表征的目标，相对于Onderstepoort，Arg24显示了四个潜在的糖基化位点。可以在Vero SLAM细胞中轻松生长的Arg24菌株的可用性，是在野生型和疫苗CDV菌株之间进行免疫和抗原比较研究的重要工具。