Introduction

In newborns, dramatic changes occur in the blood and bone marrow during the first hours; there are rapid fluctuations in the quantities of leukocytes populations. In this work, we investigated leukocytes subsets counts in two types of blood samples (cord blood and capillary blood) extracted from healthy newborns.

Methods

Blood samples from Mexican neonates were collected by Instituto Nacional de Pediatría with written informed consent. For all samples we determined leukocytes populations; neutrophils, monocytes, total lymphocytes, and populations: T CD3+ cells, TCD4+ cells, T CD8+ cells, B CD19+ cells and NK CD16+56 cells by flow cytometry. We used the Mann–Whitney U test to compare leukocytes of cord and capillary blood; also to analyze the differences between gender and we obtained reference values of the cord and capillary blood in neonates.

Results

We observed higher absolute counts and frequencies of total lymphocyte in capillary blood compared with cord blood. In absolute numbers, the capillary blood showed significant differences in neutrophils, monocytes, lymphocytes, T CD3+ cells, T CD4+ cells, T CD8+ cells, B CD19+ cells, and NK cells; no significant differences were observed between genders.

Discussion

Our data contribute to newborn Mexican reference values for all these populations of leukocytes. We found that the dispersal range differs between the two types of blood, suggesting a different fate in the immune response. Immunophenotyping of the blood cell population to identify these cells is an essential tool in the diagnosis and follow-up of neonates with immunodeficiencies and other immune disorders.

中文翻译：

---

健康墨西哥新生儿脐带和毛细血管血中白细胞和淋巴细胞的参考值。

介绍

在新生儿中，最初的几个小时内血液和骨髓中发生了巨大变化。白细胞数量迅速波动。在这项工作中，我们调查了从健康新生儿中提取的两种类型的血样（脐带血和毛细血管血）中的白细胞亚群计数。

方法

墨西哥国家儿科研究所在书面知情同意下收集了墨西哥新生儿的血样。对于所有样品，我们确定了白细胞的数量。中性粒细胞，单核细胞，总淋巴细胞和种群：通过流式细胞术检测T CD3 +细胞，TCD4 +细胞，T CD8 +细胞，B CD19 +细胞和NK CD16 + 56细胞。我们使用Mann-Whitney U检验比较了脐带血和毛细血管血的白细胞。还分析了性别之间的差异，我们获得了新生儿脐带血和毛细血管血的参考值。

结果

与脐带血相比，我们观察到毛细血管血中总淋巴细胞的绝对计数和频率更高。从绝对数量上看，毛细血管血液在中性粒细胞，单核细胞，淋巴细胞，T CD3 +细胞，T CD4 +细胞，T CD8 +细胞，B CD19 +细胞和NK细胞中显示出显着差异。性别之间没有观察到显着差异。

讨论区

我们的数据有助于所有这些白细胞种群的墨西哥新生儿参考值。我们发现两种血液之间的扩散范围不同，表明免疫反应的命运不同。对具有免疫缺陷和其他免疫疾病的新生儿进行诊断和随访，对血细胞群体进行免疫分型以鉴定这些细胞是必不可少的工具。