Introduction and Objectives

Moths are a significant source of indoor and outdoor aeroallergens. High prevalence of IgE-mediated sensitization was demonstrated in a group of patients with allergic respiratory diseases. There are no studies on adult stage of these moth species allergens involved in allergic respiratory reactions - the aim of this study.

Material and Methods

36 participants were included in an experimental study, submitted to skin prick test with Bombyx mori wing extract and six other common allergens, as well as Western blot analysis with incubated nitrocellulose membrane impregnated with silkworm moth extract and human IgE-antibody. The participants were divided into 3 groups: 1) 21 allergic patients whose skin prick test was positive to Bombyx mori wing extract, 2) eight allergic patients whose skin prick test was positive to mite and negative to Bombyx mori extract 3) seven negative non-allergic subjects.

Results

Among the 21 participants from group 1, 19 serum samples reacted to Bombyx mori extract by Western blot. All of them reacted to a protein at 80 kDa and five other proteins (66, 50, 45, 37 and 30 kDa) were identified in more than 50% of the individuals tested, considered as major allergenic proteins. Sera from seven out of eight patients sensitized to house dust mite demonstrated IgE-reactivity to Bombyx mori extract by Western blot analysis. Serum samples from healthy participants did not react at all.

Conclusion

Six major reactive proteins by immunoblot analysis from moth’s wings sensitized patients can be potential allergens. The one at 80 kDa is the major protein, seen in all IgE-reactive patients from group 1 and in none from group 2, yet to be identified. Future studies should be conducted to better characterize these proteins.

中文翻译：

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蚕蛾（Bombyx mori）与呼吸道过敏性疾病相关的主要过敏蛋白的鉴定

简介和目标

飞蛾是室内和室外空气过敏原的重要来源。在一组患有过敏性呼吸道疾病的患者中证明了 IgE 介导的致敏的高流行率。没有关于这些蛾类过敏原在过敏性呼吸反应中的成虫阶段的研究 - 本研究的目的。

材料与方法

36 名参与者参与了一项实验研究，使用家蚕翅提取物和其他六种常见过敏原进行皮肤点刺试验，以及使用浸渍蚕蛾提取物和人 IgE 抗体的硝酸纤维素膜进行蛋白质印迹分析。参与者分为 3 组：1) 21 名过敏患者的皮肤点刺试验对家蚕翅提取物呈阳性，2) 8 名过敏患者的皮肤点刺试验对螨呈阳性而对家蚕提取物呈阴性 3) 7 名阴性非过敏对象。

结果

在第 1 组的 21 名参与者中，19 份血清样本通过蛋白质印迹对家蚕提取物发生反应。所有这些都与 80 kDa 的蛋白质发生反应，并且在超过 50% 的测试个体中鉴定出其他五种蛋白质（66、50、45、37 和 30 kDa），被认为是主要的过敏性蛋白质。8 名对屋尘螨敏感的患者中有 7 名的血清通过蛋白质印迹分析证明对家蚕提取物具有 IgE 反应性。来自健康参与者的血清样本根本没有反应。

结论

通过对飞蛾翅膀致敏患者的免疫印迹分析，六种主要反应蛋白可能是潜在的过敏原。80 kDa 的蛋白质是主要蛋白质，见于第 1 组的所有 IgE 反应性患者，而第 2 组的患者均未见，尚待鉴定。应该进行未来的研究以更好地表征这些蛋白质。