Reticence of α‐amylase (α‐Amy) and α‐glucosidase (α‐Glu) is needed due to their mitigation potency on the glucose absorption. In this study, the anti‐amylolytic effects of persimmon tannins (PT) on α‐Amy and α‐Glu and their interaction mechanisms were investigated. It was found that PT inhibited α‐Amy and α‐Glu with the half inhibitory concentration (IC₅₀) values of 0.35 and 0.24 mg/ml, respectively. Fluorescence and FT‐IR spectrometry results showed that PT could bind to enzymes and alter their conformations. Molecular docking showed that the structural units of PT interacted with the key sites (amino acids Glu233, Asp197, and Asp300) of α‐Amy by H‐bonds and π–π interactions, while they bound to the residues closed to the active sites of α‐Glu. The whole results implied that PT was a promising mitigator of α‐Amy and α‐Glu. It might help to understand mechanisms of glycemic response inhibition of PT and develop certain therapeutic strategies against diabetes.

中文翻译：

---

柿子在体外通过高度与没食子酸酯化的丹宁酸与其催化封闭的侧面静态结合并改变其二级结构元素，从而减轻了α-淀粉酶和α-葡萄糖苷酶的含量。

由于α-淀粉酶（α-Amy）和α-葡萄糖苷酶（α-Glu）对葡萄糖吸收的缓解作用，因此需要保持沉默。在本研究中，研究了柿单宁（PT）对α-Amy和α-Glu的抗淀粉水解作用及其相互作用机理。发现PT抑制α-Amy和α-Glu的抑制浓度为一半（IC ₅₀）值分别为0.35和0.24 mg / ml。荧光和FT-IR光谱结果表明PT可以与酶结合并改变其构象。分子对接表明，PT的结构单元通过H键和π-π相互作用与α-Amy的关键位点（氨基酸Glu233，Asp197和Asp300）相互作用，而它们与封闭在活性位点附近的残基结合。 α‐Glu。总体结果表明，PT是α-Amy和α-Glu的有希望的缓解剂。它可能有助于了解PT的血糖反应抑制机制，并开发某些针对糖尿病的治疗策略。