OBJECTIVE AND DESIGN CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel-cell adhesion, and regulates the expression of genes associated with leukocyte migration. MATERIAL Human THP-1, RAW264.7 and HEK293 cells. METHODS Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. RESULTS We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. CONCLUSION Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.

中文翻译：

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CHRFAM7A在体外减少单核细胞/巨噬细胞迁移和集落形成。

目的和设计CHRFAM7A是一个独特的人类基因，编码α7烟碱乙酰胆碱受体的主要负性抑制剂。我们最近显示，CHRFAM7A在人白细胞中表达，增加cel细胞粘附并调节与白细胞迁移相关的基因的表达。材料人THP-1，RAW264.7和HEK293细胞。方法在软琼脂中进行细胞迁移，细胞增殖和集落形成，以比较载体与CHRFAM7A转导的细胞的生物学活性。结果我们显示将CHRFAM7A基因传递到THP-1人单核细胞系可减少细胞迁移，减少对单核细胞趋化蛋白的趋化性，并减少软琼脂中的菌落形成。结论总而言之，