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Dual mass spectrometry as a tool to improve annotation and quantification in targeted plasma lipidomics.
Metabolomics ( IF 3.6 ) Pub Date : 2020-04-17 , DOI: 10.1007/s11306-020-01677-z Liang Gao 1 , Amaury Cazenave-Gassiot 2 , Bo Burla 1 , Markus R Wenk 2 , Federico Torta 2
Metabolomics ( IF 3.6 ) Pub Date : 2020-04-17 , DOI: 10.1007/s11306-020-01677-z Liang Gao 1 , Amaury Cazenave-Gassiot 2 , Bo Burla 1 , Markus R Wenk 2 , Federico Torta 2
Affiliation
INTRODUCTION
High quality data, based on reliable quantification and clear identification of the reported lipid species, are required for the clinical translation of human plasma lipidomic studies.
OBJECTIVE
Lipid quantification can be efficiently performed on triple quadrupole (QqQ) mass spectrometers in targeted multiple reaction monitoring (MRM) mode. However, a series of issues can be encountered when aiming at unambiguous identification and accurate quantification, including (i) resolving peaks of polyunsaturated species, (ii) discriminating between plasmanyl-, plasmenyl- and odd chain species and (iii) resolving the isotopic overlap between co-eluting lipid species.
METHODS
As a practical tool to improve the quality of targeted lipidomics studies, we applied a Dual MS platform by simultaneously coupling a reversed-phase liquid chromatography separation to a QqQ and a quadrupole-time of flight (Q-ToF) mass spectrometers. In one single experiment, this platform allows to correctly identify, by high-resolution MS and MS/MS, the peaks that are quantified by MRM.
RESULTS
As proof of concept, we applied the platform on glycerophosphocholines (GPCs) and sphingomyelins (SMs), which are highly abundant in human plasma and play crucial roles in various physiological functions. Our results demonstrated that Dual MS could provide a higher level of confidence in the identification and quantification of GPCs and SMs in human plasma. The same approach can also be applied to improve the study of other lipid classes and expanded for the identification of novel lipid molecular species.
CONCLUSIONS
This methodology might have a great potential to achieve a better specificity in the quantification of lipids by targeted lipidomics in high-throughput studies.
中文翻译:
双重质谱法可作为在目标血浆脂质组学中改善注释和定量的工具。
引言人类血浆脂质组学研究的临床翻译需要基于可靠定量和清楚识别所报告脂质种类的高质量数据。目的脂质定量可在目标多反应监测(MRM)模式下在三重四极杆(QqQ)质谱仪上高效进行。但是,在明确鉴定和准确定量化时可能会遇到一系列问题,其中包括(i)解析多不饱和物质的峰,(ii)区分异戊烯基,纤溶烯基和奇数链种类,以及(iii)解决同位素重叠之间共洗脱脂质种类。方法作为提高靶向脂质组学研究质量的实用工具,我们通过将反相液相色谱分离与QqQ和四极杆飞行时间(Q-ToF)质谱仪同时耦合来应用Dual MS平台。在一个单独的实验中,该平台允许通过高分辨率MS和MS / MS正确识别由MRM量化的峰。结果作为概念验证,我们将该平台应用于甘油磷酸胆碱(GPC)和鞘磷脂(SM),它们在人体血浆中含量很高,并且在各种生理功能中起关键作用。我们的结果表明,双重质谱可在人血浆中GPC和SM的鉴定和定量分析中提供更高的可信度。相同的方法也可以用于改善对其他脂质类别的研究,并扩展到新脂质分子种类的鉴定。
更新日期:2020-04-22
中文翻译:
双重质谱法可作为在目标血浆脂质组学中改善注释和定量的工具。
引言人类血浆脂质组学研究的临床翻译需要基于可靠定量和清楚识别所报告脂质种类的高质量数据。目的脂质定量可在目标多反应监测(MRM)模式下在三重四极杆(QqQ)质谱仪上高效进行。但是,在明确鉴定和准确定量化时可能会遇到一系列问题,其中包括(i)解析多不饱和物质的峰,(ii)区分异戊烯基,纤溶烯基和奇数链种类,以及(iii)解决同位素重叠之间共洗脱脂质种类。方法作为提高靶向脂质组学研究质量的实用工具,我们通过将反相液相色谱分离与QqQ和四极杆飞行时间(Q-ToF)质谱仪同时耦合来应用Dual MS平台。在一个单独的实验中,该平台允许通过高分辨率MS和MS / MS正确识别由MRM量化的峰。结果作为概念验证,我们将该平台应用于甘油磷酸胆碱(GPC)和鞘磷脂(SM),它们在人体血浆中含量很高,并且在各种生理功能中起关键作用。我们的结果表明,双重质谱可在人血浆中GPC和SM的鉴定和定量分析中提供更高的可信度。相同的方法也可以用于改善对其他脂质类别的研究,并扩展到新脂质分子种类的鉴定。