Abstract Background The present study analyzed the synergistic protective effect of β-alanine and taurine against myocardial ischemia/reperfusion. Myocardial infarct size, lipid peroxidation, and levels of glutathione peroxidase (Gpx), superoxide dismutase (SOD), reduced glutathione (GSH), catalase, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), reactive oxygen species (ROS), apoptosis, and the mRNA and protein expression of Janus kinase 2 (JAK2) and signal transducer and activator 3 of transcription (STAT3) were determined. The molecular docking was carried out by using AutoDock 4.2.1. Results Combined treatment with β-alanine and taurine reduced myocardial infarct size, lipid peroxidation, inflammatory marker, ROS levels, and apoptosis and increased Gpx, SOD activity, GSH, and catalase activity. Furthermore, combined treatment significantly reduced JAK2 and STAT3 mRNA and protein expression compared with the control. The small molecule was docked over the SH2 domain of a STAT3, and binding mode was determined to investigate the inhibitory potential of β-alanine and taurine. β-Alanine bound to SH2 domain with ΔG of -7.34 kcal/mol and KI of 1.91 μM. Taurine bound to SH2 domain with ΔG of -7.38 kcal/mol and KI of 1.95 μM. Conclusion Taken together, these results suggest that the combined supplementation of β-alanine and taurine should be further investigated as an effective therapeutic approach in achieving cardioprotection in myocardial ischemia/reperfusion. How to cite: Hou X, Sun G, Gou L. Cardioprotective effect of taurine and β-alanine against cardiac disease in myocardial ischemia and reperfuson induced rats. Electron J Biotechnol 2020;45. https://doi.org/10.1016/j.ejbt.2020.04.003

中文翻译：

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牛磺酸和β-丙氨酸对心肌缺血再灌注大鼠心脏疾病的心脏保护作用

摘要 背景本研究分析了β-丙氨酸和牛磺酸对心肌缺血/再灌注的协同保护作用。心肌梗死面积、脂质过氧化和谷胱甘肽过氧化物酶 (Gpx)、超氧化物歧化酶 (SOD)、还原型谷胱甘肽 (GSH)、过氧化氢酶、肿瘤坏死因子-α (TNF-α)、白细胞介素-6 (IL-6) 的水平，测定了活性氧 (ROS)、细胞凋亡以及 Janus 激酶 2 (JAK2) 和信号转导和转录激活因子 3 (STAT3) 的 mRNA 和蛋白质表达。分子对接使用AutoDock 4.2.1进行。结果 β-丙氨酸和牛磺酸联合治疗可减少心肌梗死面积、脂质过氧化、炎症标志物、ROS 水平和细胞凋亡，并增加 Gpx、SOD 活性、GSH 和过氧化氢酶活性。此外，与对照相比，联合治疗显着降低了 JAK2 和 STAT3 mRNA 和蛋白质的表达。该小分子停靠在 STAT3 的 SH2 结构域上，确定结合模式以研究 β-丙氨酸和牛磺酸的抑制潜力。β-丙氨酸与 SH2 结构域结合，ΔG 为 -7.34 kcal/mol，KI 为 1.91 μM。牛磺酸与 SH2 结构域结合，ΔG 为 -7.38 kcal/mol，KI 为 1.95 μM。结论 综上所述，这些结果表明应进一步研究β-丙氨酸和牛磺酸联合补充作为实现心肌缺血/再灌注心脏保护的有效治疗方法。如何引用：Hou X, Sun G, Gou L. 牛磺酸和β-丙氨酸对心肌缺血再灌注诱导大鼠心脏疾病的心脏保护作用。电子 J 生物技术 2020；45。https: