Considered as an essential calcium sensor, the calcium-dependent protein kinase (CDPK) family plays a critical part in terrestrial plants' responses to both biotic and abiotic stresses. In the study, Avicennia marina was proved to have better heat tolerance than other species. A CDPK gene was cloned from mangrove species A. marina using RACE-PCR and designated as AmCDPK. By predicting and analyzing its properties, structures and expression patterns, we found that the amino acid sequence, containing a kinase domain and four EF-hand Ca2+-binding sites, shared high identity with Handroanthus impetiginosus and Sesamum indicum. Quantitative real-time PCR data analysis suggested that AmCDPK demonstrated significant up-regulation under heat stress. It is likely that AmCDPK is a versatile gene involved in various stresses, including dehydration, cold, light, defense and ABA stress responses by analyzing cis-elements. It is the first time that CDPKs from mangroves have been cloned and our results brought evidence to the effect of AmCDPK on heat stress, which is particularly important under the background of global warming.

中文翻译：

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高温下来自红树林Avicennia marina的AmCDPK的分子克隆和表达。

钙依赖性蛋白激酶（CDPK）家族被认为是必不可少的钙传感器，在陆生植物对生物和非生物胁迫的响应中起着至关重要的作用。在研究中，Avicennia码头被证明比其他物种具有更好的耐热性。使用RACE-PCR从红树林物种滨海曲霉克隆了一个CDPK基因，并命名为AmCDPK。通过预测和分析其性质，结构和表达模式，我们发现包含一个激酶结构域和四个EF-hand Ca2 +结合位点的氨基酸序列与Handroanthus impetiginosus和Sesamum indicum具有高度同一性。实时定量PCR数据分析表明，AmCDPK在热应激下显示出明显的上调。AmCDPK可能是一种涉及多种压力的通用基因，包括脱水，感冒，通过分析顺式元素对光，防御和ABA的应激反应。这是第一次从红树林中克隆CDPK，我们的结果为AmCDPK对热胁迫的影响提供了证据，这在全球变暖的背景下尤其重要。