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Dendrigraft poly-L-lysines delivery of DNA vaccine effectively enhances the immunogenic responses against H9N2 avian influenza virus infection in chickens.
Nanomedicine: Nanotechnology, Biology and Medicine ( IF 5.4 ) Pub Date : 2020-04-16 , DOI: 10.1016/j.nano.2020.102209
Kai Zhao 1 , Guangyu Rong 2 , Qiaoyang Teng 3 , Xuesong Li 3 , Hailing Lan 2 , Lu Yu 1 , Shuang Yu 1 , Zheng Jin 4 , Guangping Chen 5 , Zejun Li 3
Affiliation  

Biodegradable nanomaterials can protect antigens from degradation, promote cellular absorption, and enhance immune responses. We constructed a eukaryotic plasmid [pCAGGS-opti441-hemagglutinin (HA)] by inserting the optimized HA gene fragment of H9N2 AIV into the pCAGGS vector. The pCAGGS-opti441-HA/DGL was developed through packaging the pCAGGS-opti441-HA with dendrigraft poly-l-lysines (DGLs). DGL not only protected the pCAGGS-opti441-HA from degradation, but also exhibited high transfection efficiency. Strong cellular immune responses were induced in chickens immunized with the pCAGGS-opti441-HA/DGL. The levels of IFN-γ and IL-2, and lymphocyte transformation rate of the vaccinated chickens increased at the third week post the immunization. For the vaccinated chickens, T lymphocytes were activated and proliferated, the numbers of CD3+CD4+ and CD4+/CD8+ increased, and the chickens were protected completely against H9N2 AIV challenge. This study provides a method for the development of novel AIV vaccines, and a theoretical basis for the development of safe and efficient gene delivery carriers.

中文翻译:

Dendrigraft聚-L-赖氨酸DNA疫苗的递送有效增强了鸡对H9N2禽流感病毒感染的免疫原性反应。

可生物降解的纳米材料可以保护抗原免于降解,促进细胞吸收并增强免疫反应。通过将H9N2 AIV的优化HA基因片段插入pCAGGS载体中,我们构建了一个真核质粒[pCAGGS-opti441-血凝素(HA)]。通过将pCAGGS-opti441-HA与树状移植物聚-1-赖氨酸(DGL)包装在一起,开发了pCAGGS-opti441-HA / DGL。DGL不仅保护pCAGGS-opti441-HA免受降解,而且显示出高转染效率。在用pCAGGS-opti441-HA / DGL免疫的鸡中诱导了强烈的细胞免疫应答。在免疫后第三周,接种鸡的IFN-γ和IL-2水平以及淋巴细胞转化率增加。对于接种疫苗的鸡,T淋巴细胞被激活并增殖,CD3 + CD4 +和CD4 + / CD8 +的数量增加,并且完全保护了鸡免受H9N2 AIV攻击。这项研究提供了一种开发新型AIV疫苗的方法,并为开发安全有效的基因传递载体提供了理论基础。
更新日期:2020-04-16
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