BACKGROUND Miscellaneous memory cell populations that exist before organ transplantation are crucial barriers to transplantation. In the present study, we used a skin-primed heart transplantation model in mouse to evaluate the abilities of Thalidomide (TD), alone or in combination with co-stimulatory blockade, using monoclonal antibodies (mAbs) against memory T cells and alloantibodies to prolong the second cardiac survival. RESULTS In the skin-primed heart transplantation model, TD combined with mAbs significantly prolonged the second cardiac survival, accompanied by inhibition of memory CD8+ T cells. This combined treatment enhanced the CD4+Foxp3+ regulatory T cells ratio in the spleen, restrained the infiltration of lymphocytes into the allograft, and suppressed the allo-response of spleen T cells in the recipient. The levels of allo-antibodies also decreased in the recipient serum. In addition, we detected low levels of the constitutions of the lytic machinery of cytotoxic cells, which cause allograft damage. CONCLUSIONS Our study indicated a potential synergistic action of TD in combination with with mAbs to suppress the function of memory T cells and increase the survival of second allografts in alloantigen-primed mice.

中文翻译：

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阻断共刺激分子的沙利度胺可延长同种异体抗原引发的心脏同种异体移植小鼠的存活时间。

背景技术在器官移植之前存在的各种记忆细胞群是移植的关键障碍。在本研究中，我们使用小鼠皮肤启动的心脏移植模型来评估沙利度胺 (TD) 的能力，单独或与共刺激阻断联合使用针对记忆 T 细胞的单克隆抗体 (mAb) 和同种抗体来延长第二心脏存活。结果在皮肤启动的心脏移植模型中，TD联合单克隆抗体显着延长了第二次心脏存活时间，同时抑制了记忆CD8+ T细胞。这种联合治疗提高了脾脏中CD4+Foxp3+调节性T细胞的比例，抑制了淋巴细胞向同种异体移植物的浸润，并抑制了受体脾脏T细胞的同种异体反应。受体血清中同种异体抗体的水平也降低了。此外，我们检测到细胞毒性细胞裂解机制的低水平构成，这会导致同种异体移植物损伤。结论 我们的研究表明 TD 与 mAb 的组合具有潜在的协同作用，可抑制记忆 T 细胞的功能并增加同种异体抗原致敏小鼠的第二个同种异体移植物的存活率。