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Dissimilatory nitrate reduction to ammonium dominates soil nitrate retention capacity in subtropical forests
Biology and Fertility of Soils ( IF 6.5 ) Pub Date : 2020-04-15 , DOI: 10.1007/s00374-020-01457-w
Xiuzhen Shi , Jianqing Wang , Christoph Müller , Hang-Wei Hu , Ji-Zheng He , Juntao Wang , Zhiqun Huang

The abundance, community composition and activity of key nitrogen (N)-cycling functional guilds were monitored at a site where coniferous Cunninghamia lanceolate and broadleaved Mytilaria laosensis were planted in 1993. Leaf cellulose, litter C/N ratio, leaf dry matter content, soil inorganic N content were significantly higher under C. lanceolata , while specific leaf area, litter production, litter magnesium, soil C and soil C/NO 3 − ratio were higher under M. laosensis . The 15 N tracing experiment together with quantitative PCR revealed that autotrophic nitrification rates, as well as the abundances of ammonia-oxidizing archaea and comammox Nitrospira were significantly higher under C. lanceolate than M. laosensis . However, M. laosensis exhibited substantially higher nitrate retention capacity via dissimilatory nitrate reduction to ammonium (DNRA), accompanied by a significantly higher abundance of nrfA gene than C. lanceolate . The Illumina sequencing indicated that tree species markedly affected soil bacterial community composition regardless of the soil layers. Redundancy analysis suggested that litter C/N ratio was the most influential factor explaining functional gene abundances and bacterial communities. Taken together, our findings showed that M. laosensis improved soil N retention capacity mainly through inhibiting autotrophic nitrification while enhancing DNRA activity. This study highlights the importance of tree species identity in influencing the microbially-mediated N cycling and bacterial community composition.

中文翻译:

异化硝酸盐还原成铵在亚热带森林中主导土壤硝酸盐保留能力

在 1993 年种植针叶杉和阔叶松萝的地点监测了关键氮 (N) 循环功能组的丰度、群落组成和活性。 叶片纤维素、凋落物 C/N 比、叶片干物质含量、土壤C. lanceolata 下的无机氮含量显着较高,而 M. laosensis 下的比叶面积、凋落物产量、凋落物镁、土壤碳和土壤 C/NO 3 - 比值较高。15 N示踪实验结合定量PCR结果表明,披针甲自养硝化速率、氨氧化古菌和硝化螺旋藻的丰度均显着高于披针甲。然而,M。laosensis 通过异化硝酸盐还原成铵 (DNRA) 表现出显着更高的硝酸盐截留能力,伴随着比 C. lanceolate 显着更高的 nrfA 基因丰度。Illumina 测序表明,无论土壤层如何,树种都会显着影响土壤细菌群落组成。冗余分析表明凋落物C/N比是解释功能基因丰度和细菌群落的最有影响的因素。综上所述,我们的研究结果表明,M. laosensis 主要通过抑制自养硝化作用同时增强 DNRA 活性来提高土壤 N 保持能力。这项研究强调了树种特性在影响微生物介导的氮循环和细菌群落组成方面的重要性。伴随着比 C. lanceolate 显着更高的 nrfA 基因丰度。Illumina 测序表明,无论土壤层如何,树种都会显着影响土壤细菌群落组成。冗余分析表明凋落物C/N比是解释功能基因丰度和细菌群落的最有影响的因素。总之,我们的研究结果表明,M. laosensis 主要通过抑制自养硝化作用同时增强 DNRA 活性来提高土壤 N 保持能力。这项研究强调了树种特性在影响微生物介导的氮循环和细菌群落组成方面的重要性。伴随着比 C. lanceolate 显着更高的 nrfA 基因丰度。Illumina 测序表明,无论土壤层如何,树种都会显着影响土壤细菌群落组成。冗余分析表明凋落物C/N比是解释功能基因丰度和细菌群落的最有影响的因素。总之,我们的研究结果表明,M. laosensis 主要通过抑制自养硝化作用同时增强 DNRA 活性来提高土壤 N 保持能力。这项研究强调了树种特性在影响微生物介导的氮循环和细菌群落组成方面的重要性。Illumina 测序表明,无论土壤层如何,树种都会显着影响土壤细菌群落组成。冗余分析表明凋落物C/N比是解释功能基因丰度和细菌群落的最有影响的因素。总之,我们的研究结果表明,M. laosensis 主要通过抑制自养硝化作用同时增强 DNRA 活性来提高土壤 N 保持能力。这项研究强调了树种特性在影响微生物介导的氮循环和细菌群落组成方面的重要性。Illumina 测序表明,无论土壤层如何,树种都会显着影响土壤细菌群落组成。冗余分析表明凋落物C/N比是解释功能基因丰度和细菌群落的最有影响的因素。总之,我们的研究结果表明,M. laosensis 主要通过抑制自养硝化作用同时增强 DNRA 活性来提高土壤 N 保持能力。这项研究强调了树种特性在影响微生物介导的氮循环和细菌群落组成方面的重要性。laosensis 主要通过抑制自养硝化作用同时增强 DNRA 活性来提高土壤 N 保持能力。这项研究强调了树种特性在影响微生物介导的氮循环和细菌群落组成方面的重要性。laosensis 主要通过抑制自养硝化作用同时增强 DNRA 活性来提高土壤 N 保持能力。这项研究强调了树种特性在影响微生物介导的氮循环和细菌群落组成方面的重要性。
更新日期:2020-04-15
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