The main goal of this study was to perform an ecotoxicological profiling of terrestrial and aquatic cyanobacterial strains found in different soils or in toxic cyanobacterial blooms in Vojvodina region, Serbia, using the effect-directed analysis (EDA) approach. The applied procedure was based on a series of in vitro or small-scale bioassays covering multiple endpoints in combination with advanced chemical analytical protocols. Non-selective and non-target preparation techniques were used for the extraction of a broad range of chemical compounds present in three terrestrial (Anabaena Č2, Anabaena Č5, Nostoc S8) and three aquatic (Nostoc Z1, Phormidium Z2, Oscillatoria K3) strains. Ecotoxicological endpoints addressed included evaluation of the fish cytotoxicity in vitro (acute toxicity), algal growth inhibition (chronic toxicity), and interaction with cellular detoxification mechanisms. All cyanobacterial strains tested in the 1st tier EDA showed significant effects in terms of chronic toxicity and interaction with cellular detoxification. Three major fractions of different polarities were further tested in the 2nd tier, using bioassays which showed the strongest response: induction of CYP1A1 biotransformation enzyme and inhibition of zebrafish organic anion (Oatp1d1) and cation (Oct1) uptake transporters. Oscillatoria K3 strain was selected for a more detailed 3rd tier EDA, and the obtained results revealed that positive sub-fractions possess polar anion and cation compounds that are reactive to both uptake transporters, and compounds responsible for the strongest effects have a pronounced lipophilic character. Apart from lipophilic non-polar compounds that represent typical phase I substrates, sub-fractions that contained polar substances are also shown to significantly induce CYP1A1.

中文翻译：

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选定的蓝细菌菌株的生态毒理学概况分析，使用多终点效应导向分析。

这项研究的主要目标是使用效果导向分析（EDA）方法对在塞尔维亚伏伊伏丁那地区不同土壤或有毒蓝藻水华中发现的陆生和水生蓝藻菌株进行生态毒理学分析。所应用的程序基于一系列涵盖多个终点的体外或小规模生物测定，并结合了先进的化学分析方案。非选择性和非目标制备技术用于提取存在于三种陆生（AnabaenaČ2，AnabaenaČ5，Nostoc S8）和三种水生（Nostoc Z1，Pormidium Z2，Oscillatoria K3）菌株中的多种化合物。涉及的生态毒理学终点包括对鱼类体外细胞毒性（急性毒性），藻类生长抑制（慢性毒性），以及与细胞排毒机制的相互作用。在第一级EDA中测试的所有蓝细菌菌株在慢性毒性和与细胞排毒的相互作用方面均显示出显着效果。在第二层中，使用显示出最强响应的生物测定法进一步测试了三个不同极性的主要组分：CYP1A1生物转化酶的诱导和对斑马鱼有机阴离子（Oatp1d1）和阳离子（Oct1）吸收转运蛋白的抑制。选择了Oscillatoria K3菌株进行更详细的第三级EDA，获得的结果表明，正亚组分含有对两种摄取转运蛋白均具有反应性的极性阴离子和阳离子化合物，而影响最强的化合物具有明显的亲脂特性。