In this study, we evaluated the ability of waste shell powder (WSP) and moringa seed powder (MSP) individually or in combination to eliminate dye, heavy metal and resistant bacteria from the industrial wastewater (IWW). The presence of dyes, heavy metals, approximately 7.0 log CFU/ml of aerobic bacteria and 3.0–4.0 log CFU/ml of other pathogens including Escherichia coli, Pseudomonas aeruginosa, Citrobacter freundii, Serratia liquefaciens and Bacillus cereus was evident in IWW of both tannery and textile industries. In addition, depending on the type of bacterial species each bacterium was resistant to as high as 50 ppm of multi-metal (Cr⁶⁺, Cd²⁺ and Pb²⁺ or its combination) and even multi-drug resistant (amoxicillin, ampicillin, cefixime, ceftazidime, and tazobactam). Combined use of MSP (0.8 gm/100 ml IWW) and WSP (0.2 gm/100 ml IWW) treatment was able to discolor the IWW within 4 h and took 24 h to eliminate heavy metals and pathogenic bacteria to non-detectable level from the IWW, simultaneously. On the other hand, individual use of MSP or WSP was not found effective enough to remove or eliminate dye, heavy metal and bacteria simultaneously from the IWW. Similar experimental results were observed in the challenge test with laboratory-prepared effluent water containing 35.0 ppm Remazol Brilliant Blue R dye and maximum 50 ppm Cr⁶⁺. Thus, the combination of these two bio-sorbents could be applicable in IWW treatment before being discharged into the environment.

中文翻译：

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使用天然生物吸附剂去除工业废水中的染料，重金属和抗抗生素细菌

在这项研究中，我们评估了废壳粉（WSP）和辣木籽粉（MSP）单独或组合使用的能力，以消除工业废水（IWW）中的染料，重金属和耐药细菌。在两个制革厂的IWW中均明显存在染料，重金属，约7.0 log CFU / ml的好氧细菌和3.0–4.0 log CFU / ml的其他病原体，包括大肠杆菌，铜绿假单胞菌，弗氏柠檬酸杆菌，液化粘质沙雷氏菌和蜡状芽孢杆菌和纺织工业。此外，根据细菌种类的不同，每种细菌对高达50 ppm的多金属（Cr ⁶⁺，Cd ²⁺和Pb²⁺或其组合），甚至具有多重耐药性（阿莫西林，氨苄青霉素，头孢克肟，头孢他啶和他唑巴坦）。结合使用MSP（0.8 gm / 100 ml IWW）和WSP（0.2 gm / 100 ml IWW）处理能够在4 h内使IWW褪色，并花费24 h将重金属和病原细菌从体内去除至无法检测的水平。 IWW，同时。另一方面，发现单独使用MSP或WSP不能有效地同时去除或消除IWW中的染料，重金属和细菌。使用含有35.0 ppm Remazol亮蓝R染料和最大50 ppm Cr ^6+的实验室制备的污水，在挑战试验中观察到了相似的实验结果。。因此，这两种生物吸附剂的组合可在排放到环境中之前应用于IWW处理。