The present study aims to escalate the production of prophylactic agent zeaxanthin using a screened potential bacterial isolate. For this purpose, a freshwater bacterium capable of producing zeaxanthin was isolated from Bor Talav, Bhavnagar. The 16S rRNA sequence confirmed the isolate as Arthrobacter gandavensis. The bacterium was also submitted to Microbial Type Culture Collection, CSIR-Institute of Microbial Technology, Chandigarh, India, with the accession number MTCC 25325. The chemo-metric tools were employed to optimise the influencing factors such as pH, temperature, inoculum size, agitation speed, carbon source and harvest time on zeaxanthin yield. Thereafter, six parameters were narrowed down to three factors and were optimised using the central composite design (CCD) matrix. Maximum zeaxanthin (1.51 mg/g) was derived when A. gandavensis MTCC 25325 was grown under pH 6.0, 1.5% (w/v) glucose and 10% (v/v) inoculum size. A high regression coefficient (R2= 0.92) of the developed model indicated the accurateness of the tested parameters. To the best of our knowledge, this is the first report on tailoring the process parameters using chemo-metric optimisation for escalating the zeaxanthin production by A. gandavensis MTCC 25325.

中文翻译：

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甘氨酸节杆菌MTCC 25325中玉米黄质产量的改善工艺参数。

本研究旨在使用筛选出的潜在细菌分离株提高玉米黄质预防剂的生产。为此，从Bhavnagar的Bor Talav分离出能够产生玉米黄质的淡水细菌。16S rRNA序列确认分离物为甘草节杆菌。该细菌也已提交印度印度昌迪加尔CSIR-微生物技术研究所微生物类型培养物保藏中心，保藏号为MTCC25325。使用化学计量工具优化了影响因素，例如pH，温度，接种量，搅拌速度，碳源和收获时间对玉米黄质产量的影响。此后，将六个参数缩小到三个因素，并使用中央复合设计（CCD）矩阵进行了优化。当A时，最大玉米黄质（1.51 mg / g）得到了。gandavensis MTCC 25325在pH 6.0、1.5％（w / v）葡萄糖和10％（v / v）接种量的条件下生长。开发模型的高回归系数（R2 = 0.92）表明测试参数的准确性。据我们所知，这是关于使用化学计量学优化工艺参数以提高A. gandavensis MTCC 25325玉米黄质产量的第一个报告。